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ntifungal Activities of the extract from Ligusticum sinense against two Citrus Storage Pathogens

HE Lei1,2,HU Jun-hua2,,YAO Tin-shan2,LI Hong-jun2,RAN Chun2,LIU Hao-qiang2,LIU Juan1,2,LEI Hui-de2 (1 College of Horiticulture and Landscape Architecture,Southwest University,Chongqing 400716,China; 2 Citrus Research Institute,Chinese Academy of Agricultural Sciences)  
The extract from Ligusticum sinense was investigated for its bioactivity against Penicillium digitatum and Colletotrichum gloeosporioides using mycelial growth rate method.The results showed that the inhibition rates were 93.8%(3 d)and 85.4%(5 d)against P.digitatum,and 98.2%(3 d)and 91.7%(5 d)against C.gloeosporioides at 3.5 mg/mL.Bioassay of extract showed that the ethanol extract was effective to P.digitatum and C.gloeosporioides,in which the EC50 was 0.146 mg/mL(3 d),0.2109 mg/mL(5 d)and 0.1544 mg/mL(3 d),0.3222 mg/mL(5 d)respectively.Microscope observation showed that the mycelia of the two pathogens were abnormal,showing distorted,bulged,more-vacuolated,and more-branched.The results indicated that L.sinense is potentially a new botanic fungicide.
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