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《South China Fruits》 2013-01
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Development and Evaluation of A Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Huanglongbing

KONG Deying,SUN Tao,LI Yingguo,TENG Shaona,LU Lihua,DENG Zhaohui(Chongqing Entry & Exit Inspection and Quarantine Bureau,Chongqing,400020)  
Based on the conserved sequences of the outer membrane protein(OMP) gene of Candidatus Liberobacter asiaticus from GenBank,a set of the loop-mediated isothermal amplification(LAMP) primers was designed to amplify the target genes.The LAMP assay was established to detect HLB.The results showed that the detection limit of HLB-LAMP was 25 pg/μL,same as the real-time PCR method.The amplification could be conducted with a water bath within 60mins.The result could be visualized by naked eyes.This HLB-LAMP method is simple,rapid,sensitive,specific and suitable for primary workstation to detect HLB.
【Fund】: 国家质检总局科研项目(2011IK186)资助
【CateGory Index】: S436.66
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