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《South China Fruits》 2013-01
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Morphology of Pollen Grains from the Plants with Different Type of Sex by Scanning Electron Microscope(SEM) and the Viability of Pollens in Red Bayberry

JIAO Yun1,4,WANG Guoyun2,CHAI Chunyan3,JIA Huimin1,GAO Zhongshan1(1 Department of Horticulture,Zhejiang University,Hangzhou,310058;2 Yuyao South Fruit Research Institute of Zhejiang;3 Cixi Forestry Technology Extension Center of Zhejiang;4 Institute of Horticulture,Jiangsu Academy of Agricultural Sciences)  
Ten pairs of polymorphic SSR primers were used for fingerprint of three types of male flower from the plants with different sex in Cixi red bayberry,including androphyte,female individual with a mutant branch of staminate inflorescence and monoecious plant.The results showed that the female individual with a mutant branch of staminate inflorescence was the variety of Biqi,a popular cultivar.By scanning electron microscope,we found the pollen size were in the range of(22.9~23.6)×(20.3~22.1) μm,and the diameter of the three typical germinal poles were 2.90~3.03 μm without significant difference.Monoecious plant showed the largest pollen viability tested by both germination and I-KI staining.The most suitable culture media for pollen germination was 5% sucrose + 0.01% boric acid + 1% agar.
【Fund】: 公益性行业(农业)科研专项果树遗传改良与控制技术研究及其应用(200903044);; 浙江省宁波科技重点项目(2012C10012)资助
【CateGory Index】: S667.6
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