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《South China Fruits》 2013-05
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Screening of the Methods for Extraction of Total RNA from Litchi Pericarp at the Swollen Stage

ZHANG Rui;ZHOU Kaibing;Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources,Ministry of Education,Hainan University;  
The total RNA of Sanyuehong litchi pericarps were extracted at the swollen stage by improved CTAB method,SDS method,improved SDS method,improved Bugos method and TIANGEN company's RNAplant plus Reagent kit.The best extracting method was determined through comparing the extracts by detecting concentrations and purities,and RT-PCR method.The results showed that RNA of Sanyuehong litchi pericarps could be extracted by all these 5methods,and the concentrations and purities of the total RNA were significantly different from each other.The concentrations of the total RNA extracted by SDS method,improved SDS method and improved Bugos method were higher than that from other methods.The purities and integrities of the total RNA extracted by improved CTAB method and TIANGEN company's RNAplant plus Reagent kit were higher and better than that from other three methods.The RNA templates extracted by both improved CTAB method and RNAplant plus Reagent kit could be used for amplifying the fragment of 18sgene with RT-PCR.The quality of the total RNA extracted by method of improved CTAB was the best,and the total RNA was the most suitable for the subsequent operations such as reverse transcription and PCR and the construction of cDNA library.
【Fund】: 国家自然科学基金项目(31160383)资助
【CateGory Index】: S667.1
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