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《South China Fruits》 2013-05
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Study on the Causes and Prevention Methods of Vitrification in Tissue Culture of Beach Plum(Prunus maritima Marshall)

HU Suying;ZHANG Chunhong;Wang Xiaomin;WU Wenlong;LI Weilin;Institute of Botany,Chinese Academy of Sciences;  
Stem segments of beach plum(Prunus maritima Marshall)were cultured in MS medium to observe the influence of culture medium and condition on vitrification of plantlets.The results showed that the main factors leading to vitrification of beach plum plantlets were concentrations of TDZ,sucrose and agar in the culture medium,also culture temperature and photoperiod.The orthogonal experiment results showed that the impact factors on vitrification of plantlets in descending order is TDZ concentrationculture temperaturesucrose concentrationillumination time agar concentration.Based on single factor and orthogonal tests,we concluded that the optimized culture condition was at TDZ concentration of 0.5mg/L,sucrose concentration of 3%,agar concentration of 0.6%,temperature at 25℃,and irradiation time of 12~16h/d(3 000lx).This condition delivered higher proliferation coefficient of plantlets,the lower vitrification rate and the stronger growth of plant.
【Fund】: 江苏省科技支撑计划项目(BE2011324);; 江苏省农业“三新”工程项目sx(2012)411);; 江苏省/中国科学院植物研究所青年科技创新基金项目(青201101)资助
【CateGory Index】: S662
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Chinese Journal Full-text Database 10 Hits
1 YANG Li-qin,LI Rui,WANG Jun,HU Hai-ying,WU Xiao-ling(Ningxia University,Ningxia,Yinchuan 750021,China);Three Difficult Problems in Plant Tissue Culture[J];Northern Horticulture;2008-04
2 HU Shu-ying,WANG Xiao-min,ZHANG Chun-hong,WU Wen-long,LI Wei-lin,TANG Fei-yun(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014);Tissue culture with the stem of Prunus maritima Marshall[J];Journal of Anhui Agricultural University;2012-05
3 ZAI Xueming1,ZHANG Huanshi2,QIN Pei2(1 Department of Horticulture,Jinling Institute of Technology,Nanjing,210038;College of Life Science,Nanjing University);Effect of AM Fungi on Growth and Nutrient Absorption of Prunus maritima Seedlings under NaCl Stress[J];South China Fruits;2013-01
4 LI Na1,2,TAO Cheng-guang1,2,WANG Ping2,WU Zhi-gang2,ZHANG Yu-jing2(1.Chinese Academy of Agricultural Sciences,Beijing 100081;2.Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161);Studies on the Vitrification and Prevention in the Tissue Culture of Pigment Marigold[J];Northern Horticulture;2013-04
5 CAI Zuguo1 ,FU Shugeng2, HUANG Baoxiang2,DENG Xiaomei2(1.College of Agronomy, Jiangxi Agricultural University, Nanchang Jiangxi 330045,China;2.Jiangxi Academy of Forestry,Nanchang Jiangxi 330032, China);Advance of Studies on Vitrification in Plant Tissue Culture[J];Jiangxi Forestry Science and Technology;2005-02
6 LI Ya-li, ZHANG Jian, PAN Yuan-zhi(College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China);Vitrification of Shoots in Tissue Culture and Advancesin Its Prevention and Cure[J];Journal of Sichuan Agricultural University;2004-03
7 YE Tian-mou(Department of Agricalture Science,Shaoguan University,Shaoguan 512005,Guangdong,China);Advances on research of several technological puzzles during plant tissue culture[J];Journal of Shaoguan University;2010-03
8 ;影响苹果砧木M9组培苗玻璃化的因素与改善措施[J];Journal of Shandong Forestry Science and Technology;2010-05
9 YAN Dao-Liang,WANG Guang,FANG Kui,ZAI Xue-Ming,QIN Pei* Halophyte Research Laboratory,Nanjing University,Nanjing 210093,China;Tissue Culture and Rapid Propagation of Prunus maritima Marshall[J];Plant Physiology Communications;2006-05
10 WANG Xiao-min~(1,2),LI Wei-lin~(2,①),ZHAO Zhi-qiang~(3),LIANG Cheng-yuan~(2),FANG Hai-ling~(2)(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China;3.Jiangsu Science and Technology Department,Nanjing 210008,China),;Effects of different conditions of tissue culture on vitrification of Mentha haplocalyx Briq.plantlets[J];Journal of Plant Resources and Environment;2006-03
Chinese Journal Full-text Database 10 Hits
1 LI Zi-feng et al (Laiyang Agricultural University Landscape Architecture and Art,Qingdao,Shandong 266109);Research Advance in Chinese Peony Tissue Culture[J];Journal of Anhui Agricultural Sciences;2006-13
2 ZHANG Guo-qiang et al (Department of Horticulture,Xinyang Higher Agricultural School,Xinyang,Henan 464000);Development and Forecast of Plant Tissue Culture Technique in China[J];Journal of Anhui Agricultural Sciences;2006-16
3 LUO Qing et al (Research Center of Medlar Engineering and Technology,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan,Ningxia 750002);Effects of Different Culture Conditions on the Vitrification of Tissue Culture Seedlings of Lycium barbarum L.[J];Journal of Anhui Agricultural Sciences;2008-22
4 XU Mei-long et al (Key National Laboratory of Seedling Bioengineering,Yinchuan,Ningxia 750004);Study on Tissue Culture and Preventing Vitrification of Thymus quinquecostatus[J];Journal of Anhui Agricultural Sciences;2009-01
5 MA Yan et al(Forestry College,Shandong Agricultural University,Taian,Shandong 271018);Resarch Advances in the Tissue Culture of Woody Ornamental Plant[J];Journal of Anhui Agricultural Sciences;2012-04
6 NIU Wen-hao et al(College of Life Sciences,Chifeng University,Chifeng,Inner Mongolia 024000);Effects of Different Hormone Pretreatments on the Germination of Mentha arvensis Seeds[J];Journal of Anhui Agricultural Sciences;2012-21
7 HU Guo-fu,WEI Qi,HU Bao-zhong(1.Life Science Collage of Northeast Agricultural university,harbin,150030);Study on the Plant tissue culture in Coleus blumei Benth.[J];Northern Horticulture;2007-07
8 YAO Yan~1,ZHANG Ying~2,WANG Zhen-chun~1,ZENG Cui-ying~1,FAN Guo-jian~1,SHI Hai-liang~1,LI Xiu-fang~1 (1.College of Life Sciences,Guangzhou University,Guangzhou,Guangdong 510006;2.School of Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510405);Measurement of Preventing Shoot Browning of Baphicacanthus cusia(Nees)Bremek During Rapid Propagation[J];Northern Horticulture;2010-03
9 SUN Gang1,2,ZHENG Wen-gang1,QIAO Xiao-jun1,JIANG Kai1,GUO Rui1,YANG Li2(1.National Engineering Research Center for Intellgent Equipment in Agriculture,Beijing 100097;2.College of Engineering,China Agricultural University,Beijing 100083);Research Progress and Prospect of Micropropagation Robot in Plant Tissue Culture[J];Northern Horticulture;2010-15
10 LI Hui,FAN Jun-feng,GAO Jian-she,ZHOU Yong-xue,WANG Hu(College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100);Establishment of Tissue Culture Regeneration System of Populus tomentosa Clone-30[J];Northern Horticulture;2012-03
China Proceedings of conference Full-text Database 2 Hits
1 Liao feixiong Li ling Yao cuixian Guo zhongxiao (1.Floricultural research Institule,Guangdong Academy of Agricultural Science,Wushan,Guangzhou 510640; 2.College of Life,South China Normal University,Shipai,Guangzhou 510631);The Growth of Gerbera Plantlets on the Sucrose-free Medium and Effect of Different Closures on it in Vitro[A];[C];2004
2 FU Su-jing SHI Ji-qing CHEN Jian-fang ZHOU Yu-zhen (Jiangsu Provincial Forestry Seeding and Flower New & Hi-Tech Zone Wujiang City Nursery Garden Co.,Ltd,Wujiang 215222;Tongren College of Guizhou Normal University,Tongren 554300);Tissue Culture and Rapid Propagation of Prunus maritime Marsh[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MA Ji-min(Chengdu Vocational College of Agricultural Science and Technology,Chengdu,Sichuan 611130);Vitrification Phenomenon in the Tissue Culture of Eucalyptas and Its Overcoming Measures[J];Journal of Anhui Agricultural Sciences;2009-27
2 ZHANG Hong1,WANG Wan-xin2(1.Department of Agronomy,Dezhou University,Dezhou,Shandong 253023,China;2.Department of Electromechanic,Dezhou University,Dezhou,Shandong 253023,China);The Phenomenon and Preventing of Vitrification in Tissue Culture of Dianthus cargophyllus[J];Northern Horticulture;2008-08
3 DU Xue-lin~1, ZHANG Zhen-xia~2, YU Ru-gang~1, FU Yi-kun~3 (1.Huibei Coal Industry Normal College, Huibei 235000,China; 2.College of Life Science, Zhongshan University, Guangzhou 510275,China; 3.College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China);The cause of contamination and its prevention in plant tissue culture[J];Pratacultural Science;2005-01
4 TAO Ming (Shnxi Normal University,Xian 710062,China);Studies on the abnormal embryoid and hypeahydric shoot in tissue culture[J];Acta Botanica Boreali-occidentalia Sinica;2001-05
5 Zhao Jian(Institute of Materia Medica,Chinese Academca of Medicine Science,Beijing 100050)Yang Wenjie(College of Life Science,Northeast Normal University,Changchun 130024);RELATIONSHIPS OF OXYGEN FREE RADICALS STRESS AND OCCURRENCE OF VITRIFIED SHOOTS DURING MICROPROPAGATION OF PYRUS “PINGBO NO.1” (PYRUS PYRIFOLIA CULT.VAR.PINGBO NO.1) IN VITRO[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-02
6 Wu Ruojing; You Huamin;Beng Dongfei(Fujial College of Forestry, Napping 353001);Study on Vitrification of Regenerated shoots of Dianthus caryophyllus[J];JOURNAL OF FUJIAN COLLEGE OF FORESTRY;1995-04
7 ZHOU Li\|juan,JIANG Xiu\|yong (Biotechnology Center,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China);Some factors affecting vitrifiction of test\|tube plantlets of carnation (Dianthus caryophyllus L.)[J];Fujian Journal of Agricultural Sciences;1999-S1
8 ZHAO Zuo-min(An-shun Agricultural Institute, Guizhou 562109, China);Preliminary Results of Tissue Culture of Gerbera Jamesionil Bolus[J];Guizhou Agricultural Sciences;2005-03
10 Zhang Hongsheng (Yantai Fruit Research Institule. Shandong 264004) Mu Yunguan; Xin Peigang (Shandong Agricultural University, Taian 271018);STUDY ON THE MECHANISM OF VITRIFICATION OCCURED IN APPLE STEM-TIP CULTURE[J];;1991-02
China Proceedings of conference Full-text Database 1 Hits
1 FU Su-jing SHI Ji-qing CHEN Jian-fang ZHOU Yu-zhen (Jiangsu Provincial Forestry Seeding and Flower New & Hi-Tech Zone Wujiang City Nursery Garden Co.,Ltd,Wujiang 215222;Tongren College of Guizhou Normal University,Tongren 554300);Tissue Culture and Rapid Propagation of Prunus maritime Marsh[A];[C];2007
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