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《South China Fruits》 2013-05
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Study on the Causes and Prevention Methods of Vitrification in Tissue Culture of Beach Plum(Prunus maritima Marshall)

HU Suying;ZHANG Chunhong;Wang Xiaomin;WU Wenlong;LI Weilin;Institute of Botany,Chinese Academy of Sciences;  
Stem segments of beach plum(Prunus maritima Marshall)were cultured in MS medium to observe the influence of culture medium and condition on vitrification of plantlets.The results showed that the main factors leading to vitrification of beach plum plantlets were concentrations of TDZ,sucrose and agar in the culture medium,also culture temperature and photoperiod.The orthogonal experiment results showed that the impact factors on vitrification of plantlets in descending order is TDZ concentrationculture temperaturesucrose concentrationillumination time agar concentration.Based on single factor and orthogonal tests,we concluded that the optimized culture condition was at TDZ concentration of 0.5mg/L,sucrose concentration of 3%,agar concentration of 0.6%,temperature at 25℃,and irradiation time of 12~16h/d(3 000lx).This condition delivered higher proliferation coefficient of plantlets,the lower vitrification rate and the stronger growth of plant.
【Fund】: 江苏省科技支撑计划项目(BE2011324);; 江苏省农业“三新”工程项目sx(2012)411);; 江苏省/中国科学院植物研究所青年科技创新基金项目(青201101)资助
【CateGory Index】: S662
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