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《South China Fruits》 2017-01
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Clone and Construction of Knock-out Vector of CgEg1B Gene from Colletotrichum gloeosporioides on Mango

ZHANG Kaili;PU Jinji;ZHANG He;ZHOU Fangxue;YANG Yongli;LIU Yanan;LIU Xiaomei;College of Environment and Plant Protection,Hainan University/Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources/Ministry of Education Hainan University;Environment and Plant Protection Institute,CATAS/Key Laboratory of Integrated Pest Management of Tropical Crops,Ministry of Agriculture;  
Mango anthracnose was one of the most serious diseases.In order to clarify the relationship between virulence and endoglucanase gene CgEg1 B of C.gloeosporioides,CgEg1 B gene was cloned fromC.gloeosporioides from mango by method of homologous cloning.The sequence characteristics and the conservative structure domains on predicted protein of CgEg1 B gene were analyzed.The knockout vector of CgEg1 B gene was constructed by method of In-Fusion HD Cloning Kit.The results showed that the complete DNA and cDNA sequence of CgEg1 B gene were 1 077 bp and 1 020 bp in size,respectively,with one intron of 57 bp,encoding aputative protein of 339-amino acid with a molecular weight of 37.13 kDa and isoelectric point of 5.11.The CgEg1 B sequence was highly similar(99%)with endoglucanase CgEg1 B gene of C.gloeosporioides from avocado(EQB54542.1)in GenBank.The CgEg1 Bgene knockout vector pCgEg1BGH-30 was constructed.The studies suggested that CgEg1 B gene had the sequence characteristics of endoglucanase gene family,which meant that CgEg1 B might involve in invasion,implantation and pathogenicity of C.gloeosporioides.
【Fund】: 国家自然科学基金(31460455);; 公益性行业(农业)科研专项;; 杧果产业技术研究与示范(201203092-2)资助
【CateGory Index】: S436.67
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