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《Rheumatism and Arthritis》 2019-02
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Study on the Mechanism of Tiaogu Tablet(跳骨片)Regulating Wnt/β-catenin Signaling Pathway to Delay Articular Cartilage Degeneration in Osteoarthritis

HE Xiao-juan;ZHENG Wen-wei;JIA Liang-liang;LI Hui;XU Li-mei;ZENG Jian-wei;XU Hui-feng;ZHENG Chun-song;YE Jin-xia;WU Guang-wen;LI Xi-hai;YE Hong-zhi;  
Objective:To investigate the mechanism of Tiaogu Tablet(跳骨片)regulating Wnt/β-catenin signaling pathway to protect articular cartilage in osteoarthritis.Methods:Thirty two-month-old male SD rats were randomly divided into a blank control group,a model control group and a Tiaogu Tablet group,with 10 rats in each group.Animal models of osteoarthritis were established by modified Hulth method.The Tiaogu Tablet group was given Tiaogu tablet 0.32 g·kg~(-1)·d~(-1) after successful modeling.The blank control group and the model control group were given the same amount of saline by gastric lavage.After 12 weeks of intervention,the articular cartilage of rats was taken.Real-time PCR was used to detect the expressions of β-catenin,Frizzled-2,GSK-3β and CKIε mRNA in articular cartilage.Western blot was used to detect the expressions of CKIε,Collagen Ⅱ and PGS1 in articular cartilage.Massion staining method was used to observe the expressions and morphological changes of collagen in articular cartilage.Results:Real-time PCR showed that the expressions of β-catenin,Frizzled-2 and CKIε mRNA in articular cartilage of the model control group were significantly higher than those of the blank control group(P 0.05,P 0.05,P 0.05),while the expression of GSK-3β mRNA was significantly lower(P 0.05).Compared with the model control group,the expressions of β-catenin,Frizzled-2 and CKIε mRNA in articular cartilage of the Tiaogu Tablet group were significantly lower(P 0.05,P 0.05,P 0.05),while the expression of GSK-3β mRNA significantly increased(P 0.05).Western blot results showed that CKIε protein expression in articular cartilage of the model control group was significantly higher than that of the blank control group(P 0.05),while the expressions of Collagen Ⅱ and PGS1 protein were significantly lower(P 0.05,P 0.01).Compared with model control group,CKI-ε protein expression in articular cartilage of the Tiaogu Tablet group was significantly lower(P 0.05),and the expressions of Collagen Ⅱ and PGS1 protein were significantly higher(P 0.01,P 0.05).Massion results showed that the structure of articular cartilage in the blank control group and the Tiaogu Tablet tablet group was clear,the cartilage layer stained was obvious,the cartilage tissue was light blue,and the collagen content was high;while the structure of cartilage layer in the model control group was disordered,the staining was light,and the collagen content was low.Conclusion:Tiaogu Tablet can regulate Wnt/β-catenin signaling pathway to inhibit osteoarthritis inflammation,promote the synthesis of extracellular matrix and protect articular cartilage.
【Fund】: 福建省科技厅重点资助项目(2014Y0064);; 第四批全国中医(临床、基础)优秀人才研修项目(国中医药人教发[2017]24号);; 福建中医药大学校管课题-重点项目(X2016020-重点)
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 Li Yan-lin,Wang Guo-liang,Cao Bin,He Chuan,Gao Gang,Chen Wen-dong,Ma Ke,Xu Peng,Yang Guang Department of Sport Medicine,First Affiliated Hospital of Kunming Medicine College,Kunming 650031,Yunnan Province,China;Expression of type Ⅱ collagen and aggrecan in human articular cartilage tissues after blocking stromal cell derived factor-1/chemokine receptor 4 signal pathway in vitro[J];中国组织工程研究;2012-46
2 LI Xihai, LIANG Wenna, LIU Xianxiang 1. Medicine Department of Bone, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China 2. Institute of Integrated Traditional and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;The Role of Millimeter-wave Intervention for Chondrocyte in β-catenin Activity Mechanism[J];科技导报;2009-21
3 LIU Xian-xiang, LI Xi-hai, and ZHOU Jiang-tao Academy of Integrative Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou (350000);Experimental Study on Replicating Knee Osteoarthritis by Modified Hulth's Modeling Method[J];中国中西医结合杂志;2005-12
4 HUANG Ji-Han,HUANG Xiao-Hui,CHEN Zhi-Yang, ZHENG Qing-Shan,SUN Rui-Yuan Anhui Provincial Center for Drug Clinical Evaluation,Yijishan Hospital of Wannan Medical College,Wuhu 241001,Anhui, China;Dose conversion among different animals and healthy volunteers in pharmacological study[J];中国临床药理学与治疗学;2004-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhou Mingwei;Hou Jie;Zhang Qingling;Jia Yuxi;Department of Dermatology,China-Japan Union Hospital of Jilin University;Department of Nephrology,the First Hospital of Jilin University;;Effects of glycyrrhizin on repairing sciatic nerve injury in mice[J];中华实验外科杂志;2018-03
2 Lan Chao;Wang Chao;Lv Zhenyu;Liu Qi;Xu Xiaoxiang;Lv Qing;Ye Huaqing;Emergency ICU, the First Affiliated Hospital of Zhengzhou University,Key Subject of Clinical MedicineLaboratory of Colleges and Universities of Henan Province;;Effect of dopamine on hemodynamics and cerebral oxygen metabolism in the early stage of postresuscitation in rabbit with cardiac arrest[J];中华急诊医学杂志;2018-01
3 Sun Yi;Zhang Xizhe;Zhou Qi;Cao Jian;Liang Xiaodong;Song Jiannan;Bi Liwei;Li Haibo;Department of Anesthesiology,Chifeng Municipal Hospital;Department of Orthopaedics,Chifeng Municipal Hospital;;Effect of sufentanil on expression of basic fibroblast growth factor in nerve tissues after peripheral nerve injury in mice[J];中华麻醉学杂志;2017-10
4 Lu Zhuolin;Shen Xiu;Wang Hao;Liu Peixun;Wu Hongying;Zhou Zewei;Pharmaceutical Department, Tianjin Children's Hospital;Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College;;Function and safety re-evaluation of Xuebijing injection, a traditional Chinese medicine injection[J];国际生物医学工程杂志;2017-04
5 Zhao Xue;Yang Yunshuang;Zhang Rong;Kuang Zhenkun;Du Lei;Shi Pengzhan;Yu Lei;PLA Rocket Force General Hospital Training Base,Jinzhou Medical University;Department of TCM and Rehabilitation Medicine,PLA Rocket Force General Hospital;Department of Medical Affairs,PLA Rocket Force General Hospital;;The preventive and therapeutic effect of Bushen Jiedu prescription on radiation induced sex hormone disorder[J];中华放射医学与防护杂志;2017-08
6 Liu Zhenzhen;Leng Yufang;Li Xuanjie;Yang Kaiyin;Ye Yuanmei;Department of Anesthesiology,First Hospital of Lanzhou University;;Effect of oxycodone pretreatment on autophagy during renal ischemia-reperfusion in rats[J];中华麻醉学杂志;2017-03
7 Li Haibo;Sun Yi;Zhou Qi;Cao Jian;Liang Xiaodong;Song Jiannan;Bi Liwei;Zhang Xizhe;Department of Anesthesiology,,Municipal Hospital of Chifeng,Inner Mongolia Autonomous Region;;Effects of sufentanil on regeneration and repair after peripheral nerve injury in mice[J];中华麻醉学杂志;2016-11
8 Chen Tongjun;Xiang Jianmei;Wang Wei;Zhangzhou Health Vocational College;Zhangzhou Municipal Hosptial of Fujian Province;Department of Human Anatomy,Histology and Embryology,Fujian Medical University;;Effect of morinda officinalis extract on model rat with cytoxan induced testis injury[J];解剖学杂志;2016-04
9 Ma Xiaoli;Leng Yufang;Yang Peining;Ye Yuanmei;Wang Ting;Department of Anesthesiology,First Hospital of Lanzhou University;;Role of ATP-sensitive K~+ channels in oxycodone postconditioning-induced attenuation of myocardial ischemia-reperfusion injury in rats[J];中华麻醉学杂志;2016-08
10 Hua Jia;Wang Hua;Ma Peng;Gong Aihua;Jiang Peng;Department of Anesthesiology,Affiliated Hospital of Jiangsu University;Jiangsu University Medical College;;Effect of intrathecal gastrodin on skin cancer pain in mice[J];中华麻醉学杂志;2016-05
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Tan Zhilong,Xing Guosheng,Li Deda,et al.Orthopeadic Research Institute of Tianjin Hospital,Tianjin,300211;A Study of the Effects of Cytokines on Joint Chondrocyte[J];骨与关节损伤杂志;2003-05
2 JIN Dadi,SUN Wei,WANG Jixing,et al.Dep artment of Orthopaedics and Spine Surgery,Nanfang Hospital,the First Military Medical University,Guangzhou 510515,China;Potential clinical evaluation of ni tric oxide synthase inhibitor for th e treatment of osteoarthritis[J];中华骨科杂志;2002-06
3 CHEN You,SUN Cai-jiang,FANG Jian-zhen,et al.Second Hospital of Xiangya,Cent ral South University,Changsha 410011,China;Some cytokines in synovial fluid of different grade patients with knee osteoarthritis[J];中国临床康复;2002-10
4 Yan Xuesheng,Peng Yaqin,Ming Anping,et al Institute of Liver Affiliated Hospital Hubei College of Traditional Chinese Medicine 430061;An Experimental Study of the Influenece of Chuan Xiong Qin Injection Liquid on Cultilage Cell of Rabbit's Articulation[J];中国中医骨伤科杂志;2002-01
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