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《Rheumatism and Arthritis》 2019-02
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Differential Expression of lncRNA in Peripheral Blood of Rheumatoid Arthritis

LIU Jian;WAN Lei;SUN Yue;ZHANG Ping-heng;XIN Ling;JIANG Hui;FANG Yan-yan;DONG Wen-zhe;WEN Jian-ting;  
Objective:To observe the differential expression of lncRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis(RA).Methods:The RA patients(RA group)and the healthy controls(NC group)in the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine were selected,3 cases in each group.Peripheral blood of RA group and NC group were used to detect lncRNA and mRNA expression in peripheral blood mononuclear cells by high-throughput gene sequencing technology.GO and KEGG Pathway analyzed the differential expression of lncRNA function,and constructed a coexpression network of lncRNA-mRNA.Based on this,Cis and Trans predicted lncRNAs that may be associated with RA apoptosis,oxidative stress,and signaling pathways.The lncRNA was verified by RT-PCR.Results:lncRNA sequencing analysis revealed that lncRNA was significantly different between RA and NC groups.There were 9158 differentially expressed lncRNAs(differential multiples ≥ 2,and P ≤ 0.05),5682 expressions were up-regulated,3476 were down-regulated,and 7895 differentially expressed mRNAs.GO analysis found that differentially expressed mRNA is mainly involved in immune inflammatory response,apoptosis,oxidative stress,cellular metabolism,cellular transcriptional regulation,bone marrow leukocyte activation,and platelet cell activation.KEGG pathway analysis found that differentially expressed mRNA is mainly involved in rheumatoid arthritis,cellular senescence,signaling pathways and the like.RT-PCR confirmed that the lncRNAs with RA cells were ENST00000619282,ENST00000637683 and ENST00000511703.The oxidative stress was HOTAIRM1,DANCR.The Notch pathway was MALAT1,CARMN,LINC-PINT.Conclusion:Abnormal expression of lncRNA in peripheral blood lymphocytes of RA patients may be related to RA cell apoptosis,oxidative stress and signal pathway imbalance.
【Fund】: 中华人民共和国科技部中医药现代化研究(2018YFC1705200);; 安徽高校自然科学研究重点项目(KJ2017A281);; 安徽省教育厅高校自然科学研究重点项目(KJ2018A0279);; 2016年中央引导地方科技发展专项(财教[2016]1188);; 安徽省科技攻关项目(1604a0802085);; 现代中医内科应用基础与开发研究安徽省重点实验室(财教[2016]518);; 2015年技术创新服务体系专项(财教[2015]1551)
【CateGory Index】: R593.22
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