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《Rheumatism and Arthritis》 2019-02
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Determination of Total Flavonoids in Zhitong Qufeng Jiaonang(止痛祛风胶囊)by Ultraviolet-Visible Spectrophotometry

PANG Xue-feng;LIANG Guo-cheng;FANG Jian-kang;CHEN Shu-yin;JIANG Li-fen;CHEN Zhuang;FENG Yu-qing;LI Yu-ling;TANG Li-ping;HUANG Zheng-zhi;LIAO Jia-yu;  
Objective:To establish a method for the determination of total flavonoids in Zhitong Qufeng Jiaonang(止痛祛风胶囊)by ultraviolet-visible spectrophotometry.Methods:An ultrasound extraction with 70% ethanol was made for total flavonoids.The content of total flavonoids in Zhitong Qufeng Jiaonang was determined by ultraviolet-visible spectrophotometry with mangiferin as the control.The detection wavelength was 380 nm.Results:Mangiferin showed a good linear relationship between 0.001 15 and 0.009 2 mg·mL~(-1).The average recovery of mangiferin was 86.14% and RSD was 4.99%.Conclusion:The method of ultravioletvisible spectrophotometry for the determination of total flavonoids in Zhitong Qufeng Jiaonang is simple and reproducible.
【Fund】: 广西卫生厅课题(GZZJ14-03 Z2014130)
【CateGory Index】: R286.0
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