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《Radiation Protection》 2009-01
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CHANGES OF p53 RELATED GENE mRNA INDUCED BY ~(60)Coγ-RAY IN LYMPHOBLASTOID CELLS

Pan Yan Li Yuwen Feng Jiangbin Lu Xue Liu Qingjie Su Xu(National Institute for Radiation Protection,Chinese Center for Disease Control and Prevention,Beijing,100088)  
This study is to investigate the changes of GADD45 and CDKN1A gene mRNA expression induced by 60Co γ-ray in lymphoblastoid cell line in vitro,and its probability of gene expression as a biodosimeter.Lymphoblastoid cell line was irradiated by 60Co γ-ray with the dose of 0-10 Gy and cultivated for 8 hours,in order to screen the optimal dose.mRNA was extracted and real-time PCR method was used to detect the changes of PIG3 and GADD45 gene mRNA expression in lymphoblastoid cells.Lymphoblastoid cells was cultivated for 0-72 h after optimal dose irradiation.The changes of GADD45 and CDKN1A gene mRNA expression in lymphoblastoid cells in different time-point were detected.The results showed that GADD45 and CDKN1A gene expression in lymphoblastoid cells increased in a dose-dependent form.The peak expression of GADD45 was at 8 h after 5 Gy irradiation and began to decrease.The trend in change of CDKN1A gene expression was not notable.
【Fund】: 国家自然科学基金(30570551);; 卫生部卫生行业科研专项(200802018);; 公共卫生专项(20071001)
【CateGory Index】: R144
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