A primary study on deletion of HPRT in differentiated diagnosis between γ ray and benzo-pyrene
Liu Jiangong;Zhang Zhongxin;Dang Xuhong;Liu Hongyan;Wang Chao;Duan Zhikai;China Institute for Radiation Protection;
To investigate the changes of deletion of HPRT exposure to γ ray and benzo-pyrene,and to find a differential diagnosis index for cell injury induced by γ ray and benzo-pyrene human peripheral blood samples from three persons were exposed to~(60) Co γ rays at 10 different doses( 0—5 Gy) and benzopyrene at 6 different concentrations( 0—10 μg / m L),its DNA was isolated from white cell. The changes of deletion of HPRT exposure to γ ray and benzo-pyrene were detected by real time quantitative PCR.The relationship between deletion frequency of HPRT exon and irradiation dose represents certain linear correlation from 0—5 Gy. A linear regression equation has been obtained using statistics regression analysis software: exon- 2,y = 0. 360 3 + 0. 110 68 x,R~2= 0. 99,p 0. 01; exon- 5,y = 0. 429 8 +0. 082 3x,R~2= 0. 93,p 0. 01 while,the relationship between deletion frequency of HPRT and different concentrations( 0—10 μg / m L) represents no statistical significance. There is an obvious influence on deletion frequency of exon 5 of HPRT induced by γ ray,while there is no obvious influence on deletion frequency of exon 5 of HPRT induced by benzo-pyrene,which may be a new differential diagnosis index in the future.
【CateGory Index】： R440