Application of molecularly imprinted solid-phase extraction for separating and assaying amphetamines in blood samples

(CHANG Jing1,ZHU Jun1,DI Yumin2,ZHANG Leiping1,CUI Wei1,HAO Hongxia3,YU Zhongshan1 1.Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;2.Chinese People's Public Security University,Beijing 100038,China;3.Key Laboratory of Evidence Science,China University of Political Science and Law,Ministry of Education,Beijing 100088,China)  

Objecive A method for extraction of amphetamines in blood samples using molecularly imprinted solid phase extraction and gas chromatography-mass spectrometry(GC/MS).Methods Human blood samples were spiked with amphetamine,methamphetamine,MDA and MDMA,and diluted with 4∶1(v/v)with 10mmol/L ammonium acetate buffer(pH8.0);activating the MIP column with 1mL methanol and 1mL 10mmol/L ammonium acetate(pH8.0);Washing the impurities using 2 1mL water,1mL 60/40 MeCN/water and 1mL 1% HAc in MeCN;Elute the amphetamine drugs with 2 1mL 1% formic acid in methanol,then evaporate under nitrogen to dryness and reconstitute with 100μL methanol prior to GC/MS analysis.Results The recoveries obtained with this method for amphetamine drugs are more than 90%.The linear range was 20～5000ng/mL with correlation coefficients between 0.995 7 and 0.998 9.The limits of detection were 20ng/mL for amphetamine(AM),16ng/mL methamphetamine(MA),30ng/mL methylenedioxyamphetamine(MDA)and methylened ioxy methamp hetamine(MDMA),The limits of quantitative were 10ng/mL for AM,8ng/mL for methamphetamine(MAM),15ng/mL for MDA and MDMA.Conclusion Recoveries are higher,impurities were less.The method could be applied for simultaneous determination of trace amphetamines in biological specimens.

【Key Words】： forensic toxicology analysis amphetamines molecularly imprinted solid phase extraction GC/NPD GC/MS
【CateGory Index】： D919.1

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