Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Analytieal Chemistry》 1998-10
Add to Favorite Get Latest Update

Divinylbenzene-Vinylpyrrolidone Copolymer Silica Packing for Chromatographic Separation of Proteins

Yang Binghua, Fan Limin*, Wei Yun, Shen Rennan, Jiang Shengxiang, Chen Liren (Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000)  
A novel reversed-phase chromatographic packing for protein separation is described. First diethoxymethylvinylsilane was bonded to silica gel, then the divinylbenzene (DVB) and vinylpyrrolidone (VPD) were copolymerized on the silica gel to produce a packing with network - structured surface and retains the wide-pore structure of the matrix.Good selectivity and separation ability of the synthesized packing toward proteins were demonstated in the reversed-phase chromatographic mode,Seven proteins attained baseline separation within 18 min. It is able to separate biopolymer quickly.
【CateGory Index】: O658
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 XU Wen-yuan1,ZHONG Yun1,LIU Xian-liang2,HE Zhong-yi1,ZHENG Long-zhen1(1.Department of Chemistry and Chemical Engineering,East China Jiaotong University,Nanchang 330013,China;2.Editorial Board of Journal,Jiangxi Normal University,Nanchang 330027,China);Study on the alcoholysis of dichloromethylvinylsilane[J];Journal of Zhejiang University(Science Edition);2007-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Ling Fengxiang; Fan Limin; Chen Liren; Zhao Liang; Yu Weile(Lanzhou Institute of Chemical Phyics, Chinese Academy of Sciences, Lanzhou 730000);Preparation of Macroporous Silica Stationary Phase for High Performance Liquid Chromatographic Separation of Protein and Its Separation Mechanism[J];Chinese Journal of Analytieal Chemistry;1996-05
2 Li Lei, Liu Feng, Li Kean*, Sun Lei, Tong Shenyang (College of Chemistry and Molecular Engineering, Peking University, Beijing l0087l );Adsorption Behaviour of Bovine Serum Albumin on Silica Gel Bonded by Organic Reagents[J];Chinese Journal of Analytieal Chemistry;1997-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHU Li-Guo,REN Fu-Ping,CHEN Wu-LingCollege of Life Science,Northwest University,Xi'an 710069,China;Fmoc solid-phase synthesis of MSH peptide[J];Journal of the Fourth Military Medical University;2006-22
2 LI Lin,LI Jinnian,YU Weiyi(Anhui Agriculture University, Hefei ,Anhui, 230036,China);Progress in Determination Methods of the G+C Content of Bacteria[J];Progress In Veterinary Medicine;2003-01
3 SHI Zhixian\+1 HU Fengzu\+1 SHAO Yun\+1 FAN Limin\+2 \+1 (Northwest Plateau Institute of Biology, the Chinese Academy of Sciences Xining 810001) \+2 (Lanzhou Institute of Chemical physics, the Chinese Academy of Sciences Lanzhou 730000);Characteristics of Selective and Nonadsorptive of Proteins on Ion Exchange Chromatography[J];ANALYSIS AND TESTING TECHNOLOGY AND INSTRUMENTS;1999-04
4 SHI Zhi xian\+1, FAN Li min\+2 (1. Northwest Plateau Institute of Biology, the Chinese Academy of Sciences, Xining 810001, China; 2. Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 73000, China);Preparative High Performance Liquid Chromatography for Proteins[J];Analysis and Testing Technology and Instruments;2000-04
5 SONG Ya li, HU Feng zu, LIU mei, SHI Zhi xian (Northwest Plateau Institute of Biology, CAS Xining 810001; China);Analysis of Nucleosides in DNA and RNA of E.coli by RP-HPLC[J];Analysis and Testing Technology and Instruments;2002-02
6 Wei Yun; Fan Limin; Shen Runnan; Zhao Liang (Lanzhou, Institute of Chemical Physics, Chinese Academy of Sciences. Lanzhou 730000 );Preparation of a Novel Macroporous Silicagel-Polymer-Bonded Packing and Its Application to the Separation of Protein[J];Chinese Journal of Analytieal Chemistry;1996-10
7 Wei Yun, Fan Limin, Chen Liren (Lanzhou Insitute of Chemical Physiscs, Chinese Academy of Sciences, Lanzhou 730000);Effect of Different Pore Diameter Silica-based Polymer-bonded Phases on Separation of Proteins[J];Chinese Journal of Analytieal Chemistry;1997-09
8 Wei Yun, Fan Limin, Zhang Junyan, Jiang Shengxiang, Chen Liren (Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou 730000);Preparation of Silicage-Polymer - Bonded Phase and the Effect of Different Agent, pH and Salt Concentration in Elution on Proteins Separation[J];Chinese Journal of Analytieal Chemistry;1998-10
9 Wei Feng , Yang Yiwen, Ren Qilong, Wu Pingdong (National Laboratory of Secondary Resources Chemical Engineering, Zhejiang Universty, Hangzhou 310027);Preparation of Coated Cellulose Triphenylcarbamate Chiral Stationary Phase for the Resolution of Omeprazole[J];Chinese Journal of Analytical Chemistry;2003-06
10 LI Lei *, LUO Guo tian, RAO Mei xiang (Department of Chemistry, Gannan Teachers' College, Ganzhou, 341000);A Study on Adsorption Behaviour of Protein on Silica Gel Bonded by Porphyrin Reagents[J];Journal of Analytical Science;2001-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GAO Shuan-ping (Dept. Chem. & Chem. Engn., Baoji Univ. Arts & Sci., Baoji 721007, Shaanxi, China );A novel technique of separation for puerarin from Pueraria lobata (Willd) Ohwi[J];Journal of Baoji College of Arts and Science(Natural Science Edition);2004-02
2 QU Li-ping,FAN Guo-rong,MI He-ming~*,SUN Liang(Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China);Separation of soybean isoflavone glucosides from Semen Sojae Praeparatum by preparative reversed-phase high performance liquid chromatography[J];Academic Journal of Second Military Medical University;2006-03
3 Zhang Shidong Wang Jianjian Wang Xia Gao Lijuan (University of Science and Technology Liaoning,Anshan,Liao ning 114051);Isolation and classification identification of Maize Silk Monomer[J];Fujian Analysis & Testing;2007-03
4 ZHOU Tonghui~1,RUAN Wenhong~(*1),WANG Yuelin~2,RONG Minzhi~1,ZHANG Mingqiu~1(1.Materials Science Institute,Zhongshan University,Guangzhou510275, China;2.Guangzhou GBS High-Tech and Industry Co,Ltd,Guangzhou510510,China);Polypropylene composites with nano-silica modified by in-situ grafting polymerization I: Structure characterization[J];Acta Materiae Compositae Sinica;2006-02
5 Lin Lin, Wu Caiying. (Department of Chemistry, Wuhan University, Wuhan 430072);Applications of Calixarene Derivatives in Analytical Chemistry[J];Chinese Journal of Analytieal Chemistry;1997-07
6 Wei Yun, Fan Limin, Chen Liren (Lanzhou Insitute of Chemical Physiscs, Chinese Academy of Sciences, Lanzhou 730000);Effect of Different Pore Diameter Silica-based Polymer-bonded Phases on Separation of Proteins[J];Chinese Journal of Analytieal Chemistry;1997-09
7 Wang Yali; Hou Jingguo; Gao Jinzhang (Department of Chemistry, Northwest Normal University, Lanzhou 730070);Enantiomeric Separation ofα-Amino Acid Derivatives on High Performance Liquid Chromatography - Chiral Stationary Phase[J];Chinese Journal of Analytieal Chemistry;1998-12
8 Liu Yueqi; Yu Zhaowen; Jiang Shengxiang; Li Yongmin; Chen Liren(Lanzhou Institute of Chemical Physics, Chinese Academy of sciences, Lanzhou 730000)Chang Junbiao, Guo Ruiyun (Henan Province Institute of Chemistry, Zhengzhou 450002);Enantiomeric Separation of Biphenyl Dicarboxylate and Its Derivatives by High Performance Liquid Chromatography[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-03
9 Shao Baohai, Xu Xiuzhu, Lü Jiande, Fu Xiaoyun * (Department of Chemistry, Zhejiang University, Hangzhou 310027);Cellulose-based Chiral Stationary Phases for High Performance Liquid Chromatographic Enantioseparation[J];Chinese Journal of Analytical Chemistry;2003-02
10 BAI Zheng-Wu~(1,3), NG Siu-Choon~2, CHING Chi-Bun~1 (1. Chemical and Process Engineering Center, National University of Singapore, 2. Department of Chemistry, National University of Singapore, Singapore 119260; 3. Department of Chemical and Pharmaceutical Engineering, Wuhan Institute of Chemical Technology,Wuhan 430073);Synthesis and Enantioseparation Properties of a Novel Chiral Stationary Phase Derived from 1-Naphthylcarbamoylated β-Cyclodextrin[J];Journal of Analytical Science;2004-04
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 HAO Wei,XU Wen-yuan,ZHONG Yun,FANG Zhi-li,ZHOU Mei-hua,HU Lin(School of Basic Science,East China Jiaotong University,Nanchang 330013,China);A Study on the Alcoholysis of Vinyl Trichlorosilane[J];Journal of East China Jiaotong University;2008-04
2 Zhao Kun;Ji Pengyan;Liu Yin;Zhang Pengyun;Gansu Research Institute of Chemical Industry;Key Laboratory of fine Chemicals,Gansu Province;;Study on the synthesis of vinyltrifluoropropylethoxysilane[J];New Chemical Materials;2014-02
3 ZHANG Ai-xia,ZHOU Qin,CHEN Li(Chengrand Research Institute of Chemistry Industry Co.,Ltd,China National Bluestar,Chengdu 610041);Progress of Silicone Industry in China in 2007[J];Silicone Material;2008-03
4 LI Jian-yong,DENG Feng-jie (Department of Chemistry,Nanchang University,Nanchang 330031,Jiangxi);Progresses of alcoholysis of the chlorosilane[J];Silicone Material;2011-02
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Liu Feng, Li Kean, Hu Weidong, He Chiyang, Tong Shenyang(Department of Chemistry, Peking University, Beijing 100871);Enrichment and Separation of Trace Amount of Metal Ions by Silica Gel Modified With 3,6-Bis(2-arsonophenylazo)-4,5-dihydroxy-2,7-naphthalene Disulfonic Anilide[J];Chinese Journal of Analytical Chemistry;1992-04
2 Wei Yongju; Li Kean; Tong Shenyang(Department of Chemistry, Peking University, Beijing 100871);Interaction of Bromocresol Green With Serum Albumin in Acid Solution[J];Chinese Journal of Analytieal Chemistry;1996-04
3 ZHANG Yu-Guo;LIU Feng;LI Ke-An;LI Dong-Mei; WU Yan-Sheng;TONG Shen-Yang(College of Chemistry and Molecular Engineering,Peking University, Beijing,100871);Studies on Preconcentration and Separation of Trace Elements with Silica Gel Bonded by Acid Chrome Blue K[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1995-11
4 Lu Zaimin,Wu Yunliang and Cui Hufan,Research Institute of Jilin Chemical Industry Corporation,Jilin 132021.;Study on a Porous Silica Microsphere Packing for High Performance Gel Permeation Chromatography[J];Chinese Journal of Chromatography;1991-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved