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《Chinese Journal of Analytieal Chemistry》 1998-10
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Divinylbenzene-Vinylpyrrolidone Copolymer Silica Packing for Chromatographic Separation of Proteins

Yang Binghua, Fan Limin*, Wei Yun, Shen Rennan, Jiang Shengxiang, Chen Liren (Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000)  
A novel reversed-phase chromatographic packing for protein separation is described. First diethoxymethylvinylsilane was bonded to silica gel, then the divinylbenzene (DVB) and vinylpyrrolidone (VPD) were copolymerized on the silica gel to produce a packing with network - structured surface and retains the wide-pore structure of the matrix.Good selectivity and separation ability of the synthesized packing toward proteins were demonstated in the reversed-phase chromatographic mode,Seven proteins attained baseline separation within 18 min. It is able to separate biopolymer quickly.
【CateGory Index】: O658
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