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Chemiluminesencent Biosensor For Nucleic Acid

Jiang Xiongping (Institute for Drug Control of People's Liberation Army,Beijing100071) Xu Danke; Liu Yaoqing; Ma Liren (Academy of Military Medical Sciences,Beijing 100850)  
A chemiluminescent biosensor for nucleic acid was descrital. Cold surface was modfied with N-Acetylcysteine to produce a self-assembled monolayer. A single layer film of biotinylated olignucleotide probes was constructed by binding to a precursor layer of streptavidin which had been immobilized on modified gold surface covalently using carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide(NHS). A sandwich solution hybridization was executed with immobilized deoxyribo nucleic acid (DNA), target DNA and second biotinylated DNA probes. Then extravidin alkaline phosphatase which can dephosphorylate substrates AMPPD to produce stable intermediates that produce a measurable yield of light output was bound with second hybrided biotinylated DNA probes. And hepatitis B virus (HBV) DNA fragment was meased. The sensitivity of HBV DNA was obtained as 3 ×10~(-14) mol/L.
【Fund】: 国家自然科学基金!29805005
【CateGory Index】: O629
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