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《Chinese Journal of Analytical Chemistry》 2003-01
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Preparation of Temperature-sensitive Polymer with Regulated Phase Transition Temperature and Its Application in the Fluoroimmunoassay

Lin Peng 1, Zheng Hong 2, Zhang Changgong 3, Yang Huanghao 1, Li Donghui 3, Xu Jingou *1 (Department of Chemistry, The Key Laboratory of Analytical Science of Ministry of Education 1, Institute of Oceanography and Environment 2, Cancer Research Center, Xiamen University 3, Xiamen, 361005)  
A novel temperature sensitive polymer, poly(N isopropylacrylamide co acrylamide) [P(NIP co AA)], was synthesized. Its lower critical solution temperature (LCST) could be regulated by changing the content of acrylamide for different purposes. The P(NIP co AA) with phase transition temperature ( T tr )of 37℃ was used as a separating carrier for the reactants in immunoassay and a sandwich fluorescence immunoassay for rabbit IgG was established. The sensitivity of this method was comparable to that using poly N isopropylacrylamide(PNIP) as a carrier. However, owing to the elevation of T tr of the polymer, the immunoreaction could be carried out at a temperature closer to the physiological environment of organism and the rate of immunoreaction was faster. The linear range of determination was 0~1000 μg/L and the limit of detection was 10 μg/L. This method has been applied to the determination of the rabbit IgG levels in rabbit serum with satisfactory results.
【Fund】: 教育部博士点基金 (2 0 0 0 0 3 840 );; 福建省自然科学基金 (0 0 110 0 0 2 )
【CateGory Index】: O657.3
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