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《Chinese Journal of Analytical Chemistry》 2003-01
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Using Capillary Electrophoresis to Evaluate Deoxyribonucleic Acid Annealing Reaction

He Xinya, Zhou Xiaomian, Lin Bingcheng * (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023)  
Capillary Electrophoresis was used to evaluate the annealing of two complemental single stranded deoxyribonucleic acid (DNA) qualitatively and quantitatively. The annealing efficiency can be observed from the electropherogram. The migration characteristic of the double stranded DNA was explained. The NaCl concentration in the annealing DNA product matrix was determined. In general the existence of NaCl can lead the sample to dilute, but this factor could be neglected when the concentration of NaCl was 20 mmol/L in our experimental conditions.
【CateGory Index】: O658.9
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1 LIU Yuan-yuan et al (Life Science College,Lanzhou University,Lanzhou,Gansu 730000);Rapid Detection on the Enzyme-digested Products of Restriction Endonuclease by Using Capillary Electrophoresis[J];Journal of Anhui Agricultural Sciences;2008-10
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1 YANG Xin,MEI Xing-guo,WANG Ai-shun (School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074);Direct Determination of Free Aromatic Amino Acids in Suspension of Taxus chinensis Cell by High Performance Capillary Eletrophoresis[J];Amino Acids & Biotic Resources;2002-01
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