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《Chinese Journal of Analytical Chemistry》 2010-05
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Bionic Sensor for Detection of Diazepam in Meat Using Molecularly Imprinted Film

LIU Xiao-Fang, YAO Bing, LIU Guo-Yan, CHAI Chun-Yan(School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240)  
Molecularly imprinted films(MIF) were synthesized in situ using diazepam as the template on screen printed electrodes(SPE). A sensor based on MIF for the detection of diazepam was assembled by connecting the SPE to a portable conductometer through an electrode slot. Standard calibration curve was established and the detection of diazepam in meat samples was performed on this senor. Superficial characte-ristic of the MIF was investigated using scanning electron microscope. The results showed that there were numbers of imprinted micro-pores on the surface of MIF. The sensor based on MIF showed high selectivity and specificity towards the target diazepam. The response of the conductometric sensor to the concentration of diazepam displayed a linear correlation over a range of 0.039-1.25 mg/L with a detection limit of 0.008 mg/L, and the recoveries were 91.3%-95.0% based on meat samples. This method is a quick and practical approach for the detection of diazepam on sites.
【Fund】: 国家“863”计划项目(No.2006AA10Z439);; 上海市兽医生物技术重点实验室开放课题资助
【CateGory Index】: TP212
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