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《Chinese Journal of Analytical Chemistry》 2010-07
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An Indirect Inhibitive Immunoassay for Detection of Low Concentration Sulfamethoxazole in Aqueous Solution

Tang Ping-Ping1,Luo Zhao-Feng2,Cai Ji-Bao1,Su Qing-De1 1(Department of Chemistry,University of Science and Technology of China,Hefei 230026) 2(Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences,University of Science & Technology of China,Hefei 230027)  
A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed and applied to the analysis of sulfamethoxazole (SMX).A SMX MIP coating was synthesized in a 30 cm 0.25 mm i.d flexible quartz capillary by in situ polymerization technique.Thickness of the coating was 198 nm illustrated by scanning electron microscope (SEM) image.The detection was conducted on BIAcore 3000 sensor automatically.The MIP coating capillary was used as on-line solid phase extraction (SPE) tube before SPR detection.The anti-SMX mono-antibody was inhibited by SMX that was extracted by the MIP coating in a dose-dependent manner.The calibration curve was obtained by linear fit over the range of 0.04-10.00 μg/L.The detection limit was 0.01 μg/L.It was applied to the analysis of lake water samples.This method has high sensitivity and can be performed automatically.
【Fund】: 国家自然科学基金(No.20405013)资助项目
【CateGory Index】: S859.8
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1 HUANG Xiaodong, KONG Liang, LI Xin, CHEN Xueguo, GUO Baochuan, ZOU Hanfa(National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China);Preparation of Molecularly Imprinted Monolithic Capillary Column for Separation of Diastereomers of Cinchona Alkaloids by Pressure-Assisted Capillary Electrochromatography[J];Chinese Journal of Chromatography;2003-03
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