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《Chinese Journal of Analytical Chemistry》 2010-07
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An Indirect Inhibitive Immunoassay for Detection of Low Concentration Sulfamethoxazole in Aqueous Solution

Tang Ping-Ping1,Luo Zhao-Feng2,Cai Ji-Bao1,Su Qing-De1 1(Department of Chemistry,University of Science and Technology of China,Hefei 230026) 2(Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences,University of Science & Technology of China,Hefei 230027)  
A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed and applied to the analysis of sulfamethoxazole (SMX).A SMX MIP coating was synthesized in a 30 cm 0.25 mm i.d flexible quartz capillary by in situ polymerization technique.Thickness of the coating was 198 nm illustrated by scanning electron microscope (SEM) image.The detection was conducted on BIAcore 3000 sensor automatically.The MIP coating capillary was used as on-line solid phase extraction (SPE) tube before SPR detection.The anti-SMX mono-antibody was inhibited by SMX that was extracted by the MIP coating in a dose-dependent manner.The calibration curve was obtained by linear fit over the range of 0.04-10.00 μg/L.The detection limit was 0.01 μg/L.It was applied to the analysis of lake water samples.This method has high sensitivity and can be performed automatically.
【Fund】: 国家自然科学基金(No.20405013)资助项目
【CateGory Index】: S859.8
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 HUANG Xiaodong, KONG Liang, LI Xin, CHEN Xueguo, GUO Baochuan, ZOU Hanfa(National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China);Preparation of Molecularly Imprinted Monolithic Capillary Column for Separation of Diastereomers of Cinchona Alkaloids by Pressure-Assisted Capillary Electrochromatography[J];Chinese Journal of Chromatography;2003-03
2 OU Hui-chao1,2 JIANG Hao1 ZHOU Hong-min1 LI Lei-ke1 BAI Lin-jing1SONG Cun-xian2 LUO Zhao-feng1(1 Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences,University of Science & Technology of China,Hefei 230027,China)(2 The Tianjin Key Laboratory of Biomaterial Research,Institute of Biomedical Engineering,Peking Union Medical College &Chinese Academy of Medical Sciences,Tianjin 300192,China);Methods for Regeneration of Five Kinds of SPR Sensor Chips and Their Applications[J];China Biotechnology;2009-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HAN He-you~(*1,2),YOU Zi-han~1,LI Qing~1,CHEN Hao~1(1.Department of Chemistry,College of Science,Huazhong Agriculture University;2.MOA Key Laboratory of Food Safety Evaluation,Wuhan 430070);Progress in the Application of Chemiluminescence Coupled Techniques for Veterinary Drug Residue Analysis[J];Journal of Analytical Science;2005-05
2 Chi Dong Peng Xiaoxia Gong Laijin Gansu College of Chinese Medicine,Lanzhou,Gansu 730000,China;Application of Molecular Imprinting Membrane to Separating Active Components in Chinese Materia Medica[J];Guangming Journal of Chinese Medicine;2009-03
3 ZHU Li,HU Xiao-ling(Department of Chemistry,Northwest Polytechnic University,Xi'an 710072,China);The progress on mechanism of molecular imprinted chiral recognition and applied in chromatography[J];China Adhesives;2007-10
4 GU Cong-ying~1,LIN Li~2,FANG Neng-hu~(2*)(1.Department of Environmental Science and Engineering,Shanghai 200240,China;2.Department of Chemistry,Shanghai Jiao Tong University,Shanghai 200240,China);Preparation and application of monolithic column[J];Chemical Research and Application;2005-03
5 LIU You-qin,YAN Yun,XU Li,CHEN Ming-jie(Department of Applied Chemistry,College of Sciences,South China Agricultural University,Guangzhou 510642,China);Investigation and progress of chromatography column packed with molecularly imprinted polymer[J];Chemical Research and Application;2007-04
6 GAO Wen-hui1*,YANG Gui-jun1,ZHAO Wen-wei2,PEI Hong1(1.College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China; 2.Hebei Academe of Scientific and Technological Information,Shijiazhuang 050021,China);Preparation technology and application of capillary monolithic column[J];Chemical Research and Application;2009-09
7 LI Li (Light Industry Branch,Hebei Polytechnic University,Tangshan Hebei 063020,China);Application of Molecular Imprinting Technique in Pharmaceutical Science[J];Journal of Hebei Polytechnic University(Natural Science Edition);2010-02
8 OU Junjie,DONG Jing,WU Minghuo,KONG Liang,ZOU Hanfa(National Chromatographic R.& A.Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China);Applications of Molecularly Imprinted Column for Chiral Separation by High Performance Liquid Chromatography and Capillary Electrochromatography[J];Chinese Journal of Chromatography;2007-02
9 LIANG Zhe,YAO Chuan-yi(Department of Chemical Engineering and Biochemical Engineering,Xiamen University,Xiamen 361005,China);Preparation of Aminoantipyrine MIP-CEC Monolithic Column and Its Evaluation with Electrochromatography[J];Journal of Instrumental Analysis;2008-04
10 YIN Zhen-hong,CHEN Xiao-ming(Institute of Chemistry and Chemical Engineering,Xiangtan University,Xiangtan 411105,China);Synthesis and Characterization of 2,4-Dichlorophenoxyacetic Acid Molecularly Imprinted Monolithic Column and Its Chromatographic Property[J];Journal of Instrumental Analysis;2009-08
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 HUANG Xiao dong, ZOU Han fa, MAO Xi qin, LUO Quan zhou, CHEN Xiao ming, XIAO Xiang zhu (National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China);Preparation of Molecularly Imprinted Chiral Monolithic Column and Its Applications for Separation of Diastereomers[J];Chinese Journal of Chromatography;2002-05
2 LI Xue-ling1, 2 FONG Chi-chun2 CAO Hui-min1 HUANG Ming-hui2 ZHAO Jian-long1 YANG Meng-su1,2(1 Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Science, Shanghai 200050, China)(2 Biotech and Health Center, Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China);A Method for Dextran Chip Regeneration, Characterization and Application[J];China Biotechnology;2007-08
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