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《Chinese Journal of Analytical Chemistry》 2017-11
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Detection of Hep G2 Cells in Artificial Samples by Multifunctional Microfluidic Chip

ZHANG Ze-Jie;SU Xi;XU Yi;CHEN Li;School of Chemistry and Chemical Engineering,Chongqing University;International R & D center of Micro-nano Systems and New Materials Technology,Chongqing University;Key Disciplines Lab of Novel Micro-nano Devices and System Technology,Chongqing University;School of Optoelectronic Engineering,Research Center of Microsystem,Chongqing University;  
A multi-functional microfluidic chip with multi-orifice flow fractionation( MOFF) and magnetic capture technique was developed to specifically separate and capture the Hep G2 cells in artificial samples. The chip contained a glass substrate and a polydimethylsiloxane( PDMS) microchannel cover plate. The PDMS cover plate consisted of 3 injection channels of 10-mm-long,a MOFF separation zone and a hexagonal cavity cell enrichment-detection zone. Among which,the MOFF separation zone had a total length of 20 mm and was consisted of 80 semi-rhombic shrinkage/expansion units with a length of 0. 18 mm,a depth of 50 μm,a shrinkage area width of 0.06 mm,and an expansion area of 0. 20 mm. The angle between each group of shrinkage/expansion units was 103. 0°. In this experiment,Hep G2-blood cell suspension was used as the sample. Based on the principle that the magnetic bead surface-modified c-Met antibody could specifically bind to Hep G2 cells,an immunomagnetic bead( Anti-MNCs) suspension at a concentration of 50 μg/m L was prepared by surface carboxylated beads,EDC(1 mg/m L),NHS(1 mg/m L) and c-Met antibody. Under the optimized flow rate( 50 μL/min),a few Hep G2 in suspension samples were efficiently captured at the detection zone of chip via a magnetic field; the carbon quantum dots were prepared by microwave heating with citric acid and thiourea to label Hep G2 cells which achieved in-situ fluorescence visualization of captured Hep G2. Cells captured in the chip detection area were counted by microscope. The capture rate of Hep G2 cells was 88. 5% ± 6. 7%( 106 blood cells and 10 Hep G2 cells per 500 μL). The results demonstrated that the developed multifunctional microfluidic chip may serve as a promising tool for separation and capture of tumour cells.
【Fund】: 国家自然科学基金项目(No.21375156);; 国家高技术研究发展计划(863)项目(No.2015AA021104);; 重庆市前沿研究重点项目(No.cstc2015jcyj BX0010);; 重庆市科学技术委员会社会民生科技创新项目(No.cstc2015shms zx00014)资助~~
【CateGory Index】: O657.3;R735.7
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