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《Chinese Journal of Analytical Chemistry》 2017-11
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Determination of Furazolidone and Florfenicol in Soil by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

NAN Qiong;TANG Jing-Chun;HE Ruo-Zhu;HU Yu-Cheng;WU Tao;College of Environmental Science and Engineering,Nankai University;Key Laboratory of Pollution Processes and Environmental Criteria,Ministry of Education;Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation;Tianjin Hydranlic Research Insitute;  
A detection method for furazolidone and florfenicol in soil with various environmental matrices was established using ultra performance liquid chromatography-tandem spectrometry( UPLC/MS/MS) technique.Extracting solution of a mixture of phosphate buffer( p H = 3) and acetonitrile( 3 ∶ 7,V/V) was used in this experiment. The extracted water samples were enriched by SAX-HLB solid phase extraction column before the process of nitrogen blowing( high purity nitrogen). The enriched antibiotics were desalted with 8 m L of methanol. Waters BEH C18(2.1 × 100 mm) column was used for the sample separation. UPLC/MS/MS was carried out for qualitative and quantitative analysis under multi-reaction monitoring mode. The detection limit of the method was determined by 3 times of signal-to-noise ratio,and the limit of determination of the method was determined by 10 times of signal-to-noise ratio. The results showed that the detection limits of furazolidone and florfenicol were 1. 19 and 0. 41 μg/kg,respectively,and the limits of quantitation of furazolidone and florfenicol were 3. 40 and 1. 37 μg/kg,respectively. Besides,recovery experiment showed that,for the soil samples spiked with 50 μg/L furazolidone and florfenicol,the recoveries were 79% for florfenicol and 92% for furazolidone. Similarly,for the soil samples spiked with 200 μg/L furazolidone and florfenicol,the recoveries were 96% for furazolidone and 86% for florfenicol.
【Fund】: 水体污染控制与治理科技重大专项(No.2015ZX07203-011-06);; 天津市水务局局科研项目(No.KY2015-01);; 863成果转化项目(No.14RCHZSF00144)资助
【CateGory Index】: O657.63;X833
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