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Determination of γ-Linolenic Acid in Spirulina Powder by Underivatized High Performance Liguid Chromatography

MENG Di ya , REN Li wei (Food Quality Supervision and Testing Center of the Agriculture Minustry of China, Wuhan, 430064)  
An underivatized HPLC method for the determination of r linolenic acid (GLA) in spirulina powder was reported. The sample was analyzed on a C 8 reversed phase column with the mobile phase of acetonitrile tetrahydrofuran water(0.4% acetic acid) (62+3+35) using refractive index detector. GLA of a batch of spirulina was determined. The content, standard derivation, coefficient variation were 1.39%, 0.0432 and 3.11%, respectively. And the recovery ranged 93.90%~98.83%.
【CateGory Index】: O657.7
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