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A HPLC-CL Method for the Determination of Residual Sudan Ⅰ in Food

YUAN Yi-qin,ZHU Zhi-jia,REN Yi-zhen,ZHANG Wei(College of Chemistry,Chemical Engineering and Biotechnology, Donghua University,Shanghai 201620)  
A new analytical method of high performance liquid chromatography chemiluminescence(HPLC-CL) for Sudan Ⅰ is established based on the sensitizing effect of Sudan Ⅰ on luminol-KIO4 system under alkaline condition.A Phenomenex C18 column(250×4.6 mm,5 μm) as separation column and acetonitrile-methanol-water(5∶4∶1 V/V/V) as mobile phase was used throughout the experiment.The results show that a good linear relationship exists between the CL intensity and the concentration of Sudan Ⅰ in the range of 0.001~0.50 μg·mL-1(R=0.9996).The detection limit is 5 ng·kg-1 and the relative standard deviation is 1.45%(c=0.01 μg·mL-1,n=6).The recovery is in the range of 95.0%~104.0%.This method has been proved to be simple,sensitive and accurate,and it has been applied to determine Sudan Ⅰ with satisfactory results.
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