Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Study on the Interaction Between Aniline Blue and Human Serum Albumin by Fluorescence Spectroscopy

DONG Wen-juan;HOU Xiao-li;DONG Chuan;Research Center of Environmental Science and Engineering,Shanxi University;  
The interaction between aniline blue(AnB)and human serum albumin(HSA)was investigated by fluorescence spectroscopy.The results of fluorescence titration revealed that AnB could strongly quench the intrinsic fluorescence of HSA and the quenching mechanism was suggested as static quenching.The hydrophobic interaction and electrostatic force played a major role in stabilizing the complex.The binding constants and the number of binding site,corresponding thermodynamic parameters△H and △S at three different temperatures were calculated.The effect of AnB on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved