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《Molecular Plant Breeding》 2003-01
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The SNP Analysis of the Conserved Sequences of Ethylene Receptor Gene in Five Tropical Fruits

Han Jicheng 2,3 Fang Xuanjun 1,2* 1 Hainan Provincial Institute of Tropic Agriculture Resource, Sanya, 572025 2 Hainan Provincial Key Laboratory of Crop Molecular Breeding, Sanya, 572025 3 Changli Institute of Pomology, Hebei Academic of Agriculture and Forest, Changli, 066600  
The universal primer for amplifying conserved sequences of the ethylene receptor genes of five tropical fruits have been designed based on registered ethylene receptor genes (ers) in GenBank. The length of the conserved sequence from lytchee(Litch chinensis), mango(Mangifera indica) and wax apple(Syzygium samarangense) are 1440bp, and that from banana(Musa AAA Group) and papaya(Carica papaya) are 1441bp and 1439bp, respectively. Sequence analysis shows that these fragments have the 99% identities with the sequence of ethylene receptor gene (ers) of rice (Oryza Sativa), and 88% identities with that of maize (Zea mays). The sequence length polymorphism exits in one or two base pairs in obtained sequences. Each conserved sequence has 2 introns by comparing with the sequences registered in GenBank, the length of intron II is the same of 94bp in all tested tropical fruits. There are 2bp difference in intron I of banana and papaya, which the length is 373bp and 371bp, respectively. There are no difference in intron I of lytchee, mango and wax apple, which the length is 372bp. The single nucleotide variations were found in the conserved sequences of five tested tropic fruits. There are 19 SNPs (single nucleotide polymorphisms) in the obtained sequence, 12 SNPs of them in the introns, 7 SNPs of them in the encoding region. Lytchee has 4 SNPs in the encoding region more than that of others. The variation of SNPs in encoding region results in the changes of amino acids except the SNP site No.783.
【CateGory Index】: Q943
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