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《Molecular Plant Breeding》 2015-01
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Sequence Alignment on exon2-exon4 Interval of waxy Gene in Foxtail Millets

Cheng Xiaomin;Li Shutian;Yang Zili;Sun Yuyan;Liu Bin;Zhang Liyuan;Wang Siqing;Wang Dandan;Guo Shihua;Agricultural College, Inner Mongolia Agricultural University;Inner Mongolia Chifeng Agriculture and Animal Husbandry Science Institute;  
To make cl ear waxy gene sequence variances of Chinese spring foxtail millet, fifty-three millet cultivars and lines were used for sequence alignment analysis on exon2-exon4 interval of waxy gene by PCR amplification product sequencing. The results were as follows, the frequency of no-waxy, low amylase content and waxy types were 22.6%, 54.7% and 9.4%, respectively. In no-waxy millets, base deletion and substitution were detected in 11 th, 12 th, 22 ndand 23rdcultivars, but base deletion in 1st, 13 th, 18 thand 27th. In low amylase content millets, base insertion, deletion and substitution occurred in 9th, 17 thand 24thcultivars, deletion and substitution occurred in both 14 thand 15th, base insertion and deletion were in Dahonggu(00009836), base substitution and deletion were associated with 20 thand 43rd, respectively. In waxy millet, base insertion, deletion and substitution occurred in Hongniangu(00000537), but base deletion and substitution all detected in Xinghuangnian. 53 millet accessions were clusterd into ten categories at threshold value 78. There were 24 and 10 cultivars in the first and the fifth group, respectively, the latter had the most abundant SNP polymorphisms among the ten categories.
【Fund】: 国家自然科学基金项目(31260328);; 内蒙古自治区自然科学基金项目(2014MS0321);; 内蒙古自治区科技计划项目(20140165)共同资助
【CateGory Index】: S515
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Chinese Journal Full-text Database 1 Hits
1 LUO Yan-qing 1,ZU Feng 1,ZHOU Pi-cai2,ZHANG Guo-jian3,WANG Jing-qiao1,DONG Yun-song 1(1 Economic Crop Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;2 Chuxiong Institute of Agricultural Science,Chuxiong,Yunnan 675000,China;3 Yunnan Vocational and Technical College of Agriculture,Kunming 650212,China);Cluster analysis of the Ogura male sterile lines and fertility restorer lines in Brassica napus using SSR markers[J];Journal of Southern Agriculture;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 YANG Tianyu1,2,DOU Quanwen1,SHEN Yuhu1,HUANG Xiangguo1,HE Jihong2,WU Guozhong2(1 Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xi'ning, 810001,China;2 Crop Institute, Gansu Provincial Academy of Agricultural Sciences, Lanzhou, 730070,China);Genetic variation among varieties of foxtail millet from different regions in China based on RAPD markers[J];Acta Botanica Boreali-occidentalia Sinica;2003-05
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