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《Molecular Plant Breeding》 2015-01
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Sequence Alignment on exon2-exon4 Interval of waxy Gene in Foxtail Millets

Cheng Xiaomin;Li Shutian;Yang Zili;Sun Yuyan;Liu Bin;Zhang Liyuan;Wang Siqing;Wang Dandan;Guo Shihua;Agricultural College, Inner Mongolia Agricultural University;Inner Mongolia Chifeng Agriculture and Animal Husbandry Science Institute;  
To make cl ear waxy gene sequence variances of Chinese spring foxtail millet, fifty-three millet cultivars and lines were used for sequence alignment analysis on exon2-exon4 interval of waxy gene by PCR amplification product sequencing. The results were as follows, the frequency of no-waxy, low amylase content and waxy types were 22.6%, 54.7% and 9.4%, respectively. In no-waxy millets, base deletion and substitution were detected in 11 th, 12 th, 22 ndand 23rdcultivars, but base deletion in 1st, 13 th, 18 thand 27th. In low amylase content millets, base insertion, deletion and substitution occurred in 9th, 17 thand 24thcultivars, deletion and substitution occurred in both 14 thand 15th, base insertion and deletion were in Dahonggu(00009836), base substitution and deletion were associated with 20 thand 43rd, respectively. In waxy millet, base insertion, deletion and substitution occurred in Hongniangu(00000537), but base deletion and substitution all detected in Xinghuangnian. 53 millet accessions were clusterd into ten categories at threshold value 78. There were 24 and 10 cultivars in the first and the fifth group, respectively, the latter had the most abundant SNP polymorphisms among the ten categories.
【Fund】: 国家自然科学基金项目(31260328);; 内蒙古自治区自然科学基金项目(2014MS0321);; 内蒙古自治区科技计划项目(20140165)共同资助
【CateGory Index】: S515
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5 LI Wei1*,ZHI Hui1,2*,WANG Yong-fang1,LI Hai-quan1 and DIAO Xian-min1,2 1 National Millet Improvement Center/Institute of Millet Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,P.R.China 2 National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Institute of Crop Science,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,P.R.China;Assessment of Genetic Relationship of Foxtail Millet with Its Wild Ancestor and Close Relatives by ISSR Markers[J];农业科学学报(英文版);2012-04
6 ZHU Cai-mei,ZHANG Jing (National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm & Biotechnology,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081);Single Nucleotide Polymorphism of Wx Gene Associated with Amylose Content in Barley Germplasm[J];Scientia Agricultura Sinica;2010-05
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【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 HAO Xiao-fen,WANG Jie-zhi,WANG Lu-ying,WANG Gen-quan (Millet Research Institute Shanxi Academy of Agricultural,Changzhi 046011,China);Genetic Diversity of Millet by SSR Marker[J];Journal of Shanxi Agricultural Sciences;2005-04
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