Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2015-01
Add to Favorite Get Latest Update

Screening on Regeneration Capacity of Embryonic Tip from Different Genotypes of Adzuki Bean(Vigna angularis) and Optimizing on Culture Condition

Cao Jinhong;Mu Shaomeng;He Xue;Zhao Bo;Yang Kai;Li Yisong;Li Weiyu;Wan Ping;College of Plant Science and Technology, Beijing University of Agriculture, Beijing Key Laboratory of New Technology in Agricultural Application;Huairou District Agricultural Technology Extension Station;  
Efficient regenerating accessions and optimized culture condition were screen by using embryonic tips explants of 47 adzuki beans in different genotypes and different hormone concentration ratio of BAP and IBA.Regeneration rate and the average number of regenerated shoots among different genotypes of adzuki bean and media were analyzed by LSD multiple comparisons. The results showed that regenerative capacity of different genotypes in adzuki bean had obvious differences and interaction between genotypes and culture media was significantly different as well. Nine accessions with higher regenerating ability and insensitive to the medium,which included DR033, DR158, DR177, Jingnong5, DR088, DR074, Jingnong21, DR039, and DR049 were screened out with their average numbers of regenerated buds being 3.79, 3.33, 4.06, 2.93, 2.73, 2.46, 2.70, 2.32, and2.35, respectively. The regeneration rates were calculated as 92.48%, 87.04%, 80.69%, 94.15%, 96.21%, 100%,88.35%, 84.02%, and 84.67%, respectively. The regeneration cycle of embryonic tip spanned only 40 days from and embryonic tip planted in the medium to 3~5 cm cluster shoots harvested. The hormone concentration propor tion with 0.2 mg/L 6-BA +0.2 mg/L IBA exhibited a higher universality for bud induction of embryonic tip explants. The optimal hygromycin B selecting concentration for DR158, Jingnong5, Jingnong6, and Jingnong21 was7.5 mg/L, that of DR177 and DR033 were 5.0 mg/L and 10.00 mg/L, respectively. Efficient regenerating system of embryonic tip in adzuki bean was constructed in this reseach. It might facilitate practical studies for transformation and gene functional analysis of adzuki bean.
【Fund】: 国家自然科学基金项目(31272238)资助
【CateGory Index】: S521
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Jin Xingbiao Yang Cheng Chu Cheng Department of Biology;Plant Regeneration From Epicotyl Explants of Phaseolus Angularis Wight[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1985-01
2 Jin Wenlin;Yuzo Futsuhara;The Induction of Calli from AdzukiBean and Direct Plant Regeneration[J];Journal of Beijing Agricultural College;1993-01
3 ZHANG Dong-xu~1 ZHANG Jie~1 SHANG Lei~1 ZHANG Yun-wei~1 YANG Chun-yan~2 WANG Dong-mei~1 (1.College of Life Sciences,Agricultural University of Hebei,Baoding 071001,China;2.Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,China);Study on the regeneration system of soybean embryonic tips[J];Journal of Agricultural University of Hebei;2008-04
4 YAN Fan,SUN Xin,ZHAI Ying,LI Xiao-wei,WANG Ying,LI Jing-wen,WANG Qing-yu(College of Plant Science,Jilin University,Changchun 130062,Jilin,China);Effect of Different 6-BA Concentration and Genotypes on Shoots Induced from Embryonic Tips[J];Soybean Science;2011-01
5 XU Nuo,ZHANG Jun,WANG Pi-wu(Biotechnology Center,Jilin Agricultural University,Changchun 130118,Jilin,China);Effect of 6-BA and IBA Concentration on Shoots Induction from Embryonic Tips of Four Soybean Genotypes[J];Soybean Science;2012-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YAO Yan et al(College of Life Science,Shihezi University,Shihezi,Xinjiang 832003);Study on the Improvement of Embryonic Tip Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2007-21
2 SUN Wen-li et al (College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110161);Advance in Research on the Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2008-16
3 Nie Liuwang (Department of Biology);Study on the Induction and Maintenance of Calli of Phaseolus Aureus[J];Journal of Anhui Normal University(Natural Science);1990-03
4 Liu Qing Li Xinda Wang Yumin Sun Chunyu Zhang Meiping Wang Yi;Studies on Transformation of F3H Gene Interfered by RNAi in Soybean[J];Beijing Agriculture;2010-15
5 Jin Wenlin;Yuzo Futsuhara;The Induction of Calli from AdzukiBean and Direct Plant Regeneration[J];Journal of Beijing Agricultural College;1993-01
6 WANG Zhen-yi,WU Li-zhu,WANG Xing-fen,ZHANG Gui-yin,LIU Jian-feng,MA Zhi-ying (North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Key Laboratory of Crop Germplasm Resources of Hebei,Agricultural University of Hebei,Baoding 071001,China);The genetic transformation of embryonic tip via particle bombardment in cotton[J];Journal of Agricultural University of Hebei;2010-03
7 Wu Yingjie,Jiang Bo,Zhang Yan,Li Yanbang,He Lin,Wang Yucheng (Key Laboratory of Forest Tree Genetic Breeding and Biotechnology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China);Transient Expression in Tobacco by Agrobacterium-mediated Transformation[J];Journal of Northeast Forestry University;2010-09
8 Luo Zhenfeng Li Qiyun (Northeast Agricultural University, Haerbin 150030;Jilin Academy of Agricultural Sciences, Gongzhuling 136100);COMMERCIALIZATION STATUS AND RESEARCH PROGRESS OF TRANSGENIC SOYBEAN[J];Soybean Science;2005-03
9 Xue Rengao Xie Hongfeng (Laiyang Agricultural College,Qingdao 266109);Rapid And Efficient Selection For Transgenic Soybean Plants With The Improved Glufosinate Selection System[J];Soybean Science;2006-04
10 MA Xiao-hong,YAO Lu-ming,WU Tian-long(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China);High Frequency Plant Regeneration from Whole Cotyledonary Node Explants and Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean [Glycine max(L.)Merrill][J];Soybean Science;2008-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YAO Yan et al(College of Life Science,Shihezi University,Shihezi,Xinjiang 832003);Study on the Improvement of Embryonic Tip Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2007-21
2 Jin Wenlin;Jiang Lingqiu;STUDIES ON THE GROWTH AND DEVELOPMENT OF ADZUKI BEAN (Vigna angularis Ohwi and Ohashi) Ⅵ.Observation of the Blooming and Fruiting Arrangement on a Adzuki Bean Plant[J];Journal of Beijing Agricultural College;1989-03
3 LI Gui-lan,QIAO Ya-ke,HAN Jin-ling,BI Yan-juan,ZHAO Shu-guang(Hebei Normal University of Science & Technology,Changli 066600,China);Influencing factors on the survival rate of transplanted plants from Agrobacterium-mediated transformation systems of soybean cotyledonary node[J];Journal of Agricultural University of Hebei;2007-02
4 ZHANG Dong-xu~1 ZHANG Jie~1 SHANG Lei~1 ZHANG Yun-wei~1 YANG Chun-yan~2 WANG Dong-mei~1 (1.College of Life Sciences,Agricultural University of Hebei,Baoding 071001,China;2.Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,China);Study on the regeneration system of soybean embryonic tips[J];Journal of Agricultural University of Hebei;2008-04
5 Xiao Wenyan Wang Lianzheng (Chinese Academy at Agticultural Sciences, Beijing, 100081);PLANTLET REGENERATION FROM PROTOPLASTS OF SOYBEAN(GLYCINE MAX L. )THROUGH EMBRYOGENESIS[J];Soybean Science;1993-03
6 Yuan Ying 1 Liu Depu 1 Zheng Peihe 1 Xu Wenjing 1 Li Hailong 2 (1. Jilin Province Key Open Labor On Agro-Biotechnology; 2. JiLin Mechanization in Agriculture School, Jilin Province);STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE[J];Soybean Science;2001-01
7 Zhou Sijun 1 Li Xichen 1 Liu Zhaojun 1 Liu Liyan 1 Yang Qingkai 2 (1 Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 2 Institute of Soybean, Northeast Agricultural University, Harbin 150030);INTRODUCTION OF Bt GENE(cryIA)INTO SOYBEAN BY Agrobacterium-MEDIATED TRANSFORMTION[J];Soybean Science;2001-03
8 Liu Beidong 1 Zhu Yanming 2 Li Haiyan 2 Yang Qian 1 (1 Department of Life Science and Engineering ,Harbin Institute of Technology, Harbin 150001; 2 Life Science Collage ,Northeast Agriculture University, Harbin 150030);STUDY ON FACTORS AFFECTING THE SOYBEAN COTYLEDONARY NODE REGENERATION[J];Soybean Science;2002-02
9 Qiu Chengxiang Wu Tianlong (Shanghai Jiaotong University,Shanghai 201101);STUDY ON 6-BA TO THE REGENERATION OF TIP SHOOT OF SOYBEAN[J];Soybean Science;2003-01
10 Wang Ping Wu Ying Wang Gang Ji Jing (Laboratory of Plant Molecule Biology, Quartermaster University, Changchun 130062);CURRENT PROGRESS ON TISSUE CULTURE OF SOYBEAN[J];Soybean Science;2003-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved