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《Molecular Plant Breeding》 2015-01
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Acquiring Transgenic Cotton Harboring Betaine Aldehyde Dehydrogenase Gene and Identifying Their Salt-tolerance

Jiang Yurong;Yuan Junjie;Chen Guolin;Zhu Shuijin;College of Agriculture & Biotechnology, Zhejiang University;School of Agricultural and Food Science, Zhejiang A&F University;  
Betaine aldehyde dehydrogenase(BADH), widely used in studies on salt tolerance of transgenic plants,is a key enzyme in the synthesis of betaine. The BADH gene cloned from Mountain Spinach was transferred into upland cotton—variety ZMS35 by pollen-tube pathway in order to obtain the transgenic cotton plants with increased salt tolerance in this research. Through the kanamycin resistance identification of cotton seedlings in the field,PCR molecular detection of marker gene NPT- Ⅱ and target gene BADH, together with southern hybridization detection, the results proved that BADH had been integrated into the cotton genome. A total of 5 transgenic plants,carrying the marker gene NPT-Ⅱ and the target gene BADH, were obtained. Based on the experiment of T2 seed germination in the 0.6% Na Cl salt pond, the result presented that the salt tolerance of transgenic plants with the seedling emergence rate as high as 63.4% had been improved obviously in comparison with the control, whose seedling emergence rate was only 2.4%. Transgenic plants obtained in this study could be used as germplasm materials for resistant breeding.
【Fund】: 国家自然科学基金项目(31301372);; 农业部转基因生物新品种培育重大专项(2011ZX08005-005);; 浙江省教育厅一般项目(Y201328631)共同资助
【CateGory Index】: S562
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