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《Molecular Plant Breeding》 2015-01
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Identification and Characterization of mi RNAs and Their Targets Associated with the Verticillium Wilt Resistance in Cotton

Zhang Yujuan;Wang Wei;Chen Jie;Liu Jubo;Xia Minxuan;Niu Yue;Shen Fafu;State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University;  
Micro RNAs(miRNAs) are a class of endogenous non-coding small RNAs in length of 19 to 25 nucleotides, which play important roles in regulation of plant stress response. To detect miRNAs related to Verticillium wilt resistance in cotton, 64 potential miRNAs were identified in cotton by mining Sanger miRNA database and the published papaers. Subsequently, the target genes and promoters of these miRNAs were predicted and analyzed by using bioinformatic approaches. Lastly, quantitative real-time PCR assays were performed to analyze the relative expression of 12 miRNAs in cotton roots. This study may pave the way for future in-depth analysis of biological action of miRNAs and provide new information for transgenic breeding for resistance to Verticillium wilt.
【Fund】: 国家转基因生物新品种培育科技重大专项课题(2008ZX005-004)资助
【CateGory Index】: S562
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Hai-feng,SUN Qi-xin* (College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China);Advances in the Research of Model Plant Brachypodium distachyon[J];Acta Botanica Boreali-Occidentalia Sinica;2012-03
2 JIANG Zhao-yuan;Northeast Agricultural Research Center of China;;The Expression of Rice MicroRNA Response to Blast Fungus Stress[J];Journal of Anhui Agricultural Sciences;2013-17
3 LIU Zhi-xiang;ZENG Chao-zhen;CHU Lei;TAN Xiao-feng;College of Life Science and Technology, Central South University of Forestry and Technology;College of Forestry, Central South University of Forestry and Technology/Key Laboratory of Non-wood Forest Breeding & Cultivation,State Forestry Administration;;Evolution and function diversity of three new miRNA gene families in poplar[J];Guangdong Agricultural Sciences;2013-22
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5 Han Lu;Luan Yushi;School of Life Science and Biotechnology, Dalian University of Technology;;Progress on the Research of Plant miR168[J];Genomics and Applied Biology;2014-02
6 DU Chi;LIAO Maosen;ZHANG Xia;ZHANG Fuchun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;;Regulation of Copper-zinc Superoxide Dismutase Gene Expression by miR398 in Karelinia caspica under Salt Stress[J];Acta Botanica Boreali-Occidentalia Sinica;2014-04
7 MA Hui-ling;FANG Yuan-yuan;Pratacultural College,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Sino-U.S.Centers for Grazingland Ecosystem Sustainability;;Induction of plant disease resistance and its application for disease control in creeping bentgrass[J];Acta Prataculturae Sinica;2014-05
8 Ai Jia;Li Yongguang;Wang Tao;Song Beiguang;Yang Deguang;Li Wenbin;Chinese Education Ministry's Key Laboratory of Soybean Biology(Agricultural Ministry's Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding), Soybean Research Institute, Northeast Agricultural University;Northeast Agricultural University;;Research Progress of microRNA in Abiotic Stress[J];Genomics and Applied Biology;2014-05
9 HAO Yu-fan;WANG Jun-jie;YANG Dan-dan;ZHANG Jing;YAN Xin-tian;LIANG Wei-hong;College of Life Sciences, Henan Normal University;Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province;;Role of rice microRNAs in abiotic stress responses[J];Guangdong Agricultural Sciences;2014-19
10 SUN Leng-xue;PAN Teng-fei;PAN Dong-ming;College of Horticulture,Fujian Agriculture and Forestry University;;Research Advances on Regulation of MicroRNAs on Floral Bud Differentiation[J];Journal of Anhui Agricultural Sciences;2014-28
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