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《Molecular Plant Breeding》 2015-01
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Identification and Characterization of mi RNAs and Their Targets Associated with the Verticillium Wilt Resistance in Cotton

Zhang Yujuan;Wang Wei;Chen Jie;Liu Jubo;Xia Minxuan;Niu Yue;Shen Fafu;State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University;  
Micro RNAs(miRNAs) are a class of endogenous non-coding small RNAs in length of 19 to 25 nucleotides, which play important roles in regulation of plant stress response. To detect miRNAs related to Verticillium wilt resistance in cotton, 64 potential miRNAs were identified in cotton by mining Sanger miRNA database and the published papaers. Subsequently, the target genes and promoters of these miRNAs were predicted and analyzed by using bioinformatic approaches. Lastly, quantitative real-time PCR assays were performed to analyze the relative expression of 12 miRNAs in cotton roots. This study may pave the way for future in-depth analysis of biological action of miRNAs and provide new information for transgenic breeding for resistance to Verticillium wilt.
【Fund】: 国家转基因生物新品种培育科技重大专项课题(2008ZX005-004)资助
【CateGory Index】: S562
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Chinese Journal Full-text Database 10 Hits
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