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《Molecular Plant Breeding》 2015-01
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Optimizing Two-Dimensional Gel Electrophoresis System for Root Proteins of Chinese Cabbage(Brassica rapa L. ssp. Pekinensis)

Zhao Lingling;Bi Qing;Zhou Xue;Ji Ruiqin;Feng Hui;Liaoning Provincial Key Laboratory of Genetics and Breeding of Cruciferous Vegetable Crops, Department of Horticulture, Shenyang Agricultural University;  
As one of the most popular protein approaches in laboratory, two-dimensional electrophoresis(2-DE)had been widely applied to proteomic research. In order to establish an idea 2-DE system for proteomic analysis of Chinese cabbage root and to obtain perfect 2-DE profiles, 2-DE system for Chinese cabbage root was optimized based on some key factors, such as the isoelectric focusing time, the immobilized p H gradient(IPG), sample loading amount and gel concentrations. In this research, the improved 2-DE system for the root of Chinese cabbage were come out as follows: IPG strips of 17 cm in length with the p H ranges from 4 to 7, 1 200 μg loading amount,85 000 Vh of IEF time, and 12.5% gel concentration. Following this improved system, a perfect 2-DE profile of protein spots with clear background, good reproducibility and high resolution was stable generated by staining of the dye coomassie brilliant blue. This research might facilitate to carrying out proteomic studies related to root disease and root characteristics of Chinese cabbage.
【Fund】: 国家自然科学基金(312721-57);; 辽宁省自然科学基金(2013020071)共同资助
【CateGory Index】: S634.1
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Li-juan et al (Key Laboratory for the Intensive Processing of Agricultural Products in Anhui Province, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009);Optimization of Two-dimensional Electrophoresis Technology System for Rapeseed Proteome[J];Journal of Anhui Agricultural Sciences;2011-17
2 MA Hong-yu,WANG Zhan-kui,YU Tian,YANG Li-ming,WANG Xian-sheng,MA Hao*(State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China);Two-Dimensional Electrophoresis Protocol for Root Proteomics Analysis of Orchorus olitorius[J];Acta Botanica Boreali-Occidentalia Sinica;2010-01
3 CHEN Wei1,HUANG Rong-hui1,LIN Xiao-min1,YANG Zhen-hong1,WENG Zhao-xia1,TAN Fang-lin2(1.School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China; 2.Fujian Academy of Forestry,Fuzhou,Fujian 350002,China);Optimization of Two-dimensional Polyaneylamide Gel Electrophoresis for Proteome of Kandelia candel Roots[J];Fujian Journal of Agricultural Sciences;2012-08
4 Zhou Xue Feng Hui Ji Ruiqin * Department of Horticulture, Shenyang Agricultural University, Shenyang, 110866;The Best Method for Extracting and Concentration Detecting of Proteins Used for Two-dimensional Electrophoresis from Flowering Buds of Chinese Cabbage (Brassica rapa L. ssp. pekinensis)[J];Molecular Plant Breeding;2013-02
5 WANG Zhen-hua WANG Zhi-fang CHEN Shui-sen XU Fang-sen SHI Lei National Key Laboratory of Crop Genetic Improvement/Microelement Research Centre,Huazhong Agricultural University,Wuhan 430070,China;Comparison of protein extraction methods from Brassica napus roots and optimization of two-dimensional electrophoresis[J];Journal of Huazhong Agricultural University;2011-02
6 ZHANG Chao1 GUO Bin1 QI Yang1 SUN Jie1 XUE Ke1 DAI Jia-kun2 GUAN Zheng-jun1 WEI Ya-hui1(1.Key Laboratory of Resource Biology and Biotechnology in Western China(Northwest University),Ministry of Education,School of Life Science, Northwest University,Xi'an,Shaanxi 710069;2.Enzeme Engineering Insitute of Shaanxi Academy of Sciences,Xi′an,Shaanxi 710600);ESTABLISHMENT OF TWO-DIMENSIONAL GEL ELECTROPHORESIS SYSTEM FOR LEAF PROTEINS OF HBsAg TRANSGENIC CHERRY TOMATO[J];Journal of Nuclear Agricultural Sciences;2012-08
7 WANG Shan-shana,HAO Yu-hana,REN Jing-qia,GUO Chun-shena, ZHUN Yan-shua,FAN Hai-yana,b (a.College of Bioscience and Biotechnology,b.Key Laboratory of Protected Horticulture Ministry of Education,Shenyang Agricultural University,Shenyang 110161,China);Optimization of Two-dimensional Electrophoresis for Proteome of Cucumber Root[J];Journal of Shenyang Agricultural University;2012-02
8 LI Fei1,XU Qiu-fang1,ZANG Xian-peng1,LAI Yi-yu1,CHENG Wei-shun1,XU You-ping2,*,and CAI Xin-zhong1,* (1Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China; 2Centre of Analysis and Measurement,Zhejiang University,Hangzhou 310029,China);Establishment of Two-dimensional Electrophoresis System of Tomato Cotyledons[J];Acta Horticulturae Sinica;2010-04
9 YU Chu-lang 1 , YAN Shun-ping 2 , SUN Wei-ning 2 , YANG Ling 1, * ( 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China);High-Resolution Two-Dimensional Electrophoresis for Total Proteins in Rice Roots, Leaves and Suspension Cells[J];Chinese Journal of Rice Science;2006-05
10 1GU Rui Sheng 2LIU Qun Lu 2CHEN Xue Mei 2JIANG Xiang Ning 1(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093) 2(Experimental Center of Forest Biology, Beijing Forest University, Beijin;COMPARISON AND OPTIMIZATION OF THE METHODS ON PROTEIN EXTRACTION AND SDS PAGE IN WOODY PLANTS[J];CHINESE BULLETIN OF BOTANY;1999-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li MengMeng1 et al. (College of Life Sciences,Hebei University,Baoding 071002,China);Comparison of Protein Extraction Methods from Potato Tuber[J];Anhui Agricultural Science Bulletin;2009-23
2 TIAN Yi et al (Department of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161);High Performance Capillary Electrophoresis and its Application in Analysis of Protein[J];Journal of Anhui Agricultural Sciences;2006-21
3 GAO Zheng-quan et al(School of Life Sciences,Shandong University of Technology,Zibo,Shandong 255049);Research on the Role of Hevein and Chintinase in Latex Flow in Rubber Tree[J];Journal of Anhui Agricultural Sciences;2007-09
4 CAO Ying et al(College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621002);Effect of GA_3 and IAA on Regulatory of the Dynamic Accumulation of Soluble Protein in Neosinocalamus affinins and Its Relationships with the Correlated Synthetase Activities of Lignin Biosynthesis[J];Journal of Anhui Agricultural Sciences;2008-17
5 FENG Xue et al(College of Life Science,Langfang Teachers College,Langfang,Hebei 065000);Research on the Component of Different Proteins in the Gourd-shaped and Slender Waist-shaped Gourd Seeds[J];Journal of Anhui Agricultural Sciences;2009-33
6 ZHANG Xue-mei et al(College of Forestry,Heibei Agricultural University,Baoding,Hebei 071000);Effects of Apple(Malus domestica) Style Protein on Its Pollen Germination and Growth[J];Journal of Anhui Agricultural Sciences;2012-22
7 GAO Yong-liang1,WANG Qing1,CHEN Lin-bo1,2,JIANG Chang-jun1(1.Key Laboratory of Tea Biochemistry & Biotechnology,Ministry of Education,Anhui Agricultural University,Hefei 230036; 2.Tea Research Institute,Yunnan Academy of Agricultural Science,Menghai 666201);Sifting of cold resistance protein from leaves of tea plants[J];Journal of Anhui Agricultural University;2011-03
8 XU Xiao-gang1,WANG Jun2,TONG Li-li3,LI Zhi-wen2(1.Faculty of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China;2.Management Depantment of Nanjing Mochou Lake Park,Nanjing 210006,China;3.Co1lege of Horticulture Department,Jinling Institute of Technology,Nanjing 210001,China);Studies on Changes of Soluble Proteins in the Cuttings Rooting of Malus halliana Roehne[J];Northern Horticulture;2008-01
9 ZHANG Jun-huan,WANG Yu-zhu,SUN Hao-yuan,YANG Li(Institute of Pology and Forestry,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China);Study on the Extracting and Condensing Methods of Total Proteins from the Shoot Barks of Apricot[J];Northern Horticulture;2009-11
10 Yuan Shuyan; Hu Qing; Chen Xuemei; Li Yue; Jiang Xiangning. College of Biology, Beijing For. Univ., 100083, P.R.China;Effects of jasmonic acid on salt tolerance of suspension-cultured Populus euphratica cells.[J];Journal of Beijing Forestry University;2002-03
China Proceedings of conference Full-text Database 1 Hits
1 DU Shao-hua~1 BU Zhi-guo~1 WEN Xiu-jun~2 HUANG Da-zhuang~1 LIU Jin-chuan~1 (~1 Agricultural University of Hebei ,Baoding 071000; ~2 Hebei Forest Research Institute,Shijiazhuang 050061);Analysis of Fore-and-aft Inoculated Leaf Protein in Different Resistant JWB[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Shao-bin et al(Biological Science and Technology College,Shenyang Agricultural University,Shenyang,Liaoning 110161);Application of Two-dimensional Electrophoresis Technology in the Study on Plant Proteomics[J];Journal of Anhui Agricultural Sciences;2006-10
2 QU Zhong-hua et al(Shenyang Agriculture University,Shenyang,Liaoning 110161);Preliminary Research of Cucumber Leaf Proteome after Treatment with Bio-seed Coating[J];Journal of Anhui Agricultural Sciences;2007-21
3 REN Jian-sheng et al (Bioengineering Department of Zhengzhou Unversity,Zhengzhou,Henan 450001);Comparison of Protein Sample Preparation Methods from Taxus Cell for Two-dimensional Electrophoresis[J];Journal of Anhui Agricultural Sciences;2009-25
4 WANG Li-juan et al (Key Laboratory for the Intensive Processing of Agricultural Products in Anhui Province, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009);Optimization of Two-dimensional Electrophoresis Technology System for Rapeseed Proteome[J];Journal of Anhui Agricultural Sciences;2011-17
5 Gu Ruisheng1) Liu Qunlu Chen Xuemei Jiang Xiangning2) (ExperimentalCenterof Forest Biology,College of Plant Sciences,Beijing For.Univ.,100083 ,P.R.China);An Improved Method of 2DElectrophoresisfor Protein Analysis of Woody Plants[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-05
6 WU Qing-feng~1,WANG Chong-ying~(1*),WANG Xin-yu~1,LI Zhi-xiao~(2,3),WANG Ya-fu~3(1 Institute of Cell Biology,School of Life Science,Lanzhou University,Lanzhou 730000,China;2 State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou 730000,China;3 Key Laboratory of Arid and Grassland Agroecology of Ministry of Education,Lanzhou University,Lanzhou 730000,China);Establishment of Two-dimension Electrophoresis of Leaf Proteins of Lathyrus sativus[J];Acta Botanica Boreali-Occidentalia Sinica;2006-07
7 Liu Zhanji1,2 Yang Xiaohong2 Bi Yuping1* 1 Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences, Jinan, 250100; 2 National Maize Improvement Centre, China Agricultural University, Beijing, 100094;Proteomics and its Application in the Agriculture[J];Molecular Plant Breeding;2006-S1
8 CHEN Rui-hong ZHANG Gai-sheng LIU Wei YE Jing-xiu NIU Na(Shaanxi Key Laboratory of Crop Heterosis /Wheat Breeding Engineering Research Center,Ministry of Education,Northwest A& F University,Yangling,Shaanxi 712100);OPTIMIZATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS FOR PROTEOME FROM WHEAT ANTHERS[J];Journal of Nuclear Agricultural Sciences;2008-04
9 LIU Jia-you YU Min LIU Li-ping XIAO Hong-dong(Department of Horticulture,Foshan University,Guangdong Province,Foshan 528000,China);Effect of Boron on the Content of Cell Wall Polysaccharides in Border Cells and Root Apices of Pea(Pisum sativum)[J];Journal of Huazhong Agricultural University;2009-03
10 Chu Haiyan\ Yu Min\ Wang Yunhua\ Wu Lishu\= (Huazhong Agricultural University, Wuhan 430070);STUDY ON DIFFERENCES OF BORON USE EFFICIENCY OF \=RAPE(Brassica napus L.) CULTIVARS[J];JOURNAL OF HUAZHONG AGRICULTURAL;1999-02
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