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《Molecular Plant Breeding》 2015-01
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Optimizing Two-Dimensional Gel Electrophoresis System for Root Proteins of Chinese Cabbage(Brassica rapa L. ssp. Pekinensis)

Zhao Lingling;Bi Qing;Zhou Xue;Ji Ruiqin;Feng Hui;Liaoning Provincial Key Laboratory of Genetics and Breeding of Cruciferous Vegetable Crops, Department of Horticulture, Shenyang Agricultural University;  
As one of the most popular protein approaches in laboratory, two-dimensional electrophoresis(2-DE)had been widely applied to proteomic research. In order to establish an idea 2-DE system for proteomic analysis of Chinese cabbage root and to obtain perfect 2-DE profiles, 2-DE system for Chinese cabbage root was optimized based on some key factors, such as the isoelectric focusing time, the immobilized p H gradient(IPG), sample loading amount and gel concentrations. In this research, the improved 2-DE system for the root of Chinese cabbage were come out as follows: IPG strips of 17 cm in length with the p H ranges from 4 to 7, 1 200 μg loading amount,85 000 Vh of IEF time, and 12.5% gel concentration. Following this improved system, a perfect 2-DE profile of protein spots with clear background, good reproducibility and high resolution was stable generated by staining of the dye coomassie brilliant blue. This research might facilitate to carrying out proteomic studies related to root disease and root characteristics of Chinese cabbage.
【Fund】: 国家自然科学基金(312721-57);; 辽宁省自然科学基金(2013020071)共同资助
【CateGory Index】: S634.1
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