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《Molecular Plant Breeding》 2015-09
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Sequence and Activity Analysis of Os GAD_3 Promoter from Giant Embryo Rice

Xu Ming;Zhao Shuai;Lin Zhiming;Yin Hengjie;Zhang Yuwen;Liao Sufeng;Zheng Jingui;College of Crop Science,Fujian Agriculture and Forestry University;  
Using the PCR mothed, a 959 bp upstream pomotor sequence of Os GAD3 gene was isolated from the genomic DNA of giant embryo rice variety‘Tge B', which was rich in γ-amino butyric acid(GABA). Sequence analysis indicated that the fragment contained meristem-specific expression cis-acting elements CAT-box and CCGTCC-box, and various stress-resistance cis-acting elements. The cloned promoter has been used in construction of binary vectors with GUS reporter gene, and then several transformed rice were obtained through Agrobacteriummediated transformation. The results of histochemical GUS analysis showed that there were higher GUS activities in roots and stem of transgenic rice, especially strong in root tip and primary phloem of stem and GUS expression was also detected in leaf cut side and embryo. Meanwhile, GUS expression was increased significantly after soaked in water and increased gradually during soaking. These results provide further insight into understanding the regulation of Os GAD3 expression in giant embryo rice.
【Fund】: 福建省自然科学基金项目(2012J01090);; 福建农林大学科技创新平台建设项目(ptjh12015)共同资助
【CateGory Index】: S511;Q943.2
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