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《Molecular Plant Breeding》 2015-09
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Construction and Genetic Transformation of Plant Expression Vector Harboring Insect Resistant Gene Cry1Ab-Ma in Maize

Yang Xiaoyan;Weng Jianfeng;Xie Shuzhang;Lei Kairong;Biotechnology Research Center,Chongqing Academy of Agricultural Sciences,Chongqing Key Laboratory of Adversity Agriculture Research;Institute of Crop Science,Chinese Academy of Agricultural Sciences;  
The plant expression vector p TF101.1-P35S::Cry1Ab-Ma was constructed using an In-Fusion kit. In this study, the insect resistant genes Cry1Ab-Ma was transferred into the hybrid maize HiⅡ immature embryo with the agrobacterium mediated method. Forty-three seedlings were obtained after selecting with bialaphos sodium, and 28 seedlings of them were positive by PCR analysis and detection of the Bt protein. The protein extracts of T1 generation plant were detected by Cry1 Ab immune strip, it was shown that the target gene was separated and expressed in a part of transgenic plants. Thus, this study should lay a foundation for maize inbred line breeding with favorite pest resistant.
【Fund】: 重庆市基本科研业务专项(2014cstc-jbky-00502);; 重庆市农业科学院农业发展资金基础科研项目(NKY-2014AC025);; 重庆市农发良种创新项目(NKY-2015AB011)共同资助
【CateGory Index】: S513
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