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《Molecular Plant Breeding》 2015-09
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Bioinformatical and Expression Analysis of Polygalacturonase Gene from Capsicum chinense

Shen Longbin;Liu Ziji;Cao Zhenmu;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture;  
In this study, based on the sequence information of pepper genome published on Gene Bank, the full length c DNA of PG gene was obtained from Capsicum chinense L. by RT-PCR and named CCa PG. Analysis of the CCa PG nucleotide sequence revealed that the gene comprises 1 107 nucleotides that encodes a 368 amino acid polypeptide, the bioinformatics analysis results showed that the protein is a hydrophilic protein, in the N terminal has a signal peptide. The secondary structures of CCa PG were mainly α-helix(18.75%), extended strand(37.23%),random coil(36.41%), beta turn(7.61%). With alignment and phylogenetic analysis, which indicated that CCa PG had the highest identity amino acid sequence with Capsicum annuum and Nicotiana sylvestris PG protein, respect98.37% and 76.16%. Quantitative RT-PCR analyses indicated that the expression level of CCa PG was highest in the colour-changed stage, followed by bear fruit stage and lowest in the mature stage. The results provide theoretical basis for further studying the biology function of CCa PG and regulatory mechanism of ripeness in Capsicum chinense L. fruit.
【Fund】: 海南省重点科技计划应用研究及产业化项目(ZDXM20130046);; 海南耕地改良关键技术研究与示范专项(HNGDpz2015)共同资助
【CateGory Index】: S641.3
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