Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2015-09
Add to Favorite Get Latest Update

Bioinformatical and Expression Analysis of Polygalacturonase Gene from Capsicum chinense

Shen Longbin;Liu Ziji;Cao Zhenmu;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture;  
In this study, based on the sequence information of pepper genome published on Gene Bank, the full length c DNA of PG gene was obtained from Capsicum chinense L. by RT-PCR and named CCa PG. Analysis of the CCa PG nucleotide sequence revealed that the gene comprises 1 107 nucleotides that encodes a 368 amino acid polypeptide, the bioinformatics analysis results showed that the protein is a hydrophilic protein, in the N terminal has a signal peptide. The secondary structures of CCa PG were mainly α-helix(18.75%), extended strand(37.23%),random coil(36.41%), beta turn(7.61%). With alignment and phylogenetic analysis, which indicated that CCa PG had the highest identity amino acid sequence with Capsicum annuum and Nicotiana sylvestris PG protein, respect98.37% and 76.16%. Quantitative RT-PCR analyses indicated that the expression level of CCa PG was highest in the colour-changed stage, followed by bear fruit stage and lowest in the mature stage. The results provide theoretical basis for further studying the biology function of CCa PG and regulatory mechanism of ripeness in Capsicum chinense L. fruit.
【Fund】: 海南省重点科技计划应用研究及产业化项目(ZDXM20130046);; 海南耕地改良关键技术研究与示范专项(HNGDpz2015)共同资助
【CateGory Index】: S641.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 8 Hits
1 DONG Li-li,WANG Yong-bao,LI Ying-zhi * ,LI Hong-bo,ZHANG Jia-qi,YE Chun-hai * (College of Agriculture,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China);Activity of glycosidase and polygalacturonase in jackfruit fruit[J];Journal of Southern Agriculture;2013-06
2 Shen Longbin;Shen Danyan;Niu Yu;Liu Ziji;Dang Xuanmin;Yang Yan;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture;Hunan Industry Polytechnic;;Cloning and Structure Predicting of McGS1 Gene from the Bitter Gourd(Momordica charantia L.)[J];Molecular Plant Breeding;2015-05
3 KAN Juan~1,JIN Chang-hai~1,WANG Zhi-jun~1,LU Zhao-xin~2,YU Zhi-fang~2(1.Coll of Food Sci and Engin,Yangzhou Univ,Yangzhou,225001,China;2.Coll of Food Sci and Tech,Nanjing Agric Univ,Nanjing,210095,China);The effect of β-galactosidase and polygalacturonase on peach ripening and softening[J];Journal of Yandzhou University;2006-03
4 XUE Bing-ye1,SHU Huai-rui (Shandong Agricultural University, Tai'an 271018,China);THE ROLE OF POLYGALACTURONASE (PG) IN FRUIT RIPENING[J];Journal of Shandong Agricultural University;2002-02
5 ZHANG Zhao-Qi, DUAN Xue-Wu1, LU Wang-Jin(Department of Horticulture, South China Agricultural University, Guangzhou 510642, China);Recent advance on fruit polygalacturonase in molecular biology[J];Chinese Bulletin of Life Sciences;2002-02
6 KOU Xiao-hong et al. (College of Food Science and Nutrition Engineering in China Agricultural University, Beijing 100083,China);Review of Current Research on Plant Polygalacturonase[J];Journal of Shanxi Agricultural University;2004-04
7 SONG Li-Ping~1,LIU Jin-Hui~2,ZHENG Dian-Feng~(1,*),FENG Nai-Jie~1 1 College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;2 Daqing Tadano Import Crane Large-Scale Repair Shop,Daqing,Heilongjiang 163355,China;Effects of Different Plant Growth Regulators on Abscission Rate and Polygalacturonase Activities of Soybean Flowers and Pods by Spraying in Different Stages[J];Plant Physiology Journal;2011-04
8 ZHANG Min;WANG Ai-ling;YANG Jun;DU Juan;LIAO Xin-fu;Xinjiang Grapes and Melons Research Institute;;Changes of cell wall degrading enzymes during post-harvest softening of melon 'Huangzuixian'[J];Journal of Northwest A & F University(Natural Science Edition);2015-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LUO Ai-min et al(School of Light Industry and Food,Sichuan University,Chengdu,Sichuan 610065);Research on the Effects of Different Treatments on the Enzymatic Dynamics of PG Enzyme in Juicy Peach Fruit[J];Journal of Anhui Agricultural Sciences;2009-08
2 ZAI Wen-shan et al(Wenzhou Institute of Science and Technology,Wenzhou,Zhejiang 325000);Study on Differences of Storage Performance for Mature Process of Different Tomatoes[J];Journal of Anhui Agricultural Sciences;2011-27
3 PIAO Yi-long,ZHAO Lan-hua,WU Rong-zhe(Agricultural College of Yanbian University,Longjing,Jilin 133400,China);Change in Pectin Decomposition Enzyme During Storage of Pear Fruits[J];Northern Horticulture;2009-04
4 LI Jian-li1,HAN Tao1,LI Li-ping2,SUN Yan1,ZHANG Hai-ying1 (1.Department of Food Science,Beijing University of Agricultural,Beijing 102206,China;2.Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology & Business University,Beijing 100037,China);Effects of Acetaldehyde on Fruit Softening and Pectin Metabolism in Peach at Two Temperatures[J];Journal of Beijing University of Agriculture;2009-02
5 ZHANG Bingxiu, WANG Aoxue, LI Jingfu (College of Horticulture, Northeast Agricultural University, Harbin 150030, China );Progress on cell wall hydrolase of tomato fruit[J];Journal of Northeast Agricultural University;2009-01
6 Zhang Dangquan, Tan Xiaofeng(The Key Lab of Non-timber Tree Breeding and Cultivation of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, P. R. China); Zhong Jian(National Engineering Center of Cell Products);cDNA Cloning of Polygalacturonase from Alternaria alternata[J];Journal of Northeast Forestry University;2006-06
7 CHEN Ru-gang,YANG Rui-ping,GONG Zhen-hui,LI Da-wei,SHA Hjahan(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China);Cloning and Sequence Analysis of the Polygalacturonase Gene CaPG in Pepper[J];Acta Botanica Boreali-Occidentalia Sinica;2010-10
8 WANG Zhao-yuan et al(Changli Institute of Pomology,Hebei Academy of Agriculture and Forestry Sciences,Changli,Hebei 066600);Advandce of Research on Peach Postharvest Physiology[J];Horticulture & Seed;2011-05
9 ZHOU Hui-juan1,2,QIAO Yong-jin1,2,ZHANG Shao-ling2,WANG Hai-hong2,CHEN Zhao-liang2(1Agro-product Fresh Keeping and Processing Research Center,Shanghai Academy of Agricultural Sciences,Shanghai 201106 China;2Col-lege of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China);Study on the effects of different maturity on the fruit shelf-qualities and difference in metabolism of Datuanmilu honey peach cultivar[J];Journal of Fruit Science;2010-02
10 ZENG Zhao-xu;Agricultural College of Yanbian University;;Effects of CaCl_2 Treatment on Softening of Actinidia arguta Fruit[J];Journal of Anhui Agricultural Sciences;2014-03
China Proceedings of conference Full-text Database 1 Hits
1 YIN Ke-lin~1,WEI Na~2,WEN Zhi-wei~1,HU Jia-yu~1,WANG Yang~1 (1 College of Horticulture and Landscape Architecture, Southwest University,Chongqing 400716;2 Development and Planning Department,Southwest University,Chongqing 400716);Effect of Pre-Heat Treatment on Cell Wall Degradation in Strawberry Fruit[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ying-bo et al (College of Agronomy,Hainan University,Danzhou,Hainan 571737);Cloning and Primary Analysis on NBS Type Resistance Gene Analogs in Hevea brasiliensis[J];Journal of Anhui Agricultural Sciences;2009-24
2 XU Xiao-wan et al (Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640);Research Progress on the Molecular Breeding of Capsicum annuum[J];Journal of Anhui Agricultural Sciences;2009-25
3 Xu Qiang, Cao Beisheng, Jiang Jiezeng;Effects of Nitrogen, Potassium Fertilizer and Density on Water Bamboo Yield[J];Journal of Changjiang Vegetables;2004-03
4 TIAN Xiao hong,LI Sheng xiu,WANG Zhao hui (Department of Resource and Enviromental Sciences,Northwestern Agricultural University,Yangling,Shaanxi 712100,China);Uptake capacity of ammonium and nitrate to several crops[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-01
5 WANG Quan-shuai,ZHAO Dan-ying,SHEN Lin,SHENG Ji-ping* (College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China);Regulation of Plant Organs Abscission by Abscission Regulating Substances[J];Acta Botanica Boreali-Occidentalia Sinica;2009-11
6 Xu Bingqiang Du Zhongjun Huang Junsheng Tropical Biotechnology Research Institute, China Academy of Tropical Agriculture Science, Haikou, 571101 State Key Lab for Tropical Crops Biotechnology, China Academy of Tropical Agriculture Science, Haikou, 571101 Entironment And Plant Protection Research Institute, China Academy of Tropical Agriculture Science, Danzhou, 573717;Advances in the Cloning Candidate Disease Resistant Genes with the RGA Cloning Method[J];Molecular Plant Breeding;2004-03
7 Duan Xuewu, Zhang Zhaoqi,and Ji Zuoliang ( Deptartment of Horticulture, South China Agricultural University, Guangzhou,Guangdong 510642);Advances in Research on the Relationship Between Polygalcturonase and Fruit Softening[J];Journal of Fruit Science;2001-04
8 He Hongmen;Qin Zhiwei;Feng Zhuo;Wu Tao;Xin Ming;Zhou Xiuyan;Horticulture College of Northeast Agricultural Univrsity;;Cloning and Expression Analysis of Cucumber Oligopeptide Tansporter Gene under Low Nitrogen Conditions[J];Molecular Plant Breeding;2013-05
9 JIN Changhai1,WANG Zhijun1,LU Zhaoxin2,MIZUNO Masashi3,TERAI Hirofumi3,TSUCHIDA Hironobu3(1College of Food Science and Engineering,Yangzhou University ,Yangzhou225009, China;2College of Food Science and Technolege,Nanjing Agricultural University,Nanjing 210095,China;3.Laboratory of Hort Postharvest Science,Faculty of Agricultural,Kobe University,Kobe 6578501,Japan);Purification and Dynamics Property of β-Galactosidase Isozyme of Apple[J];Jiangsu Journal of Agricultural Sciences;2003-01
10 Lü Shuang-shuang1,LI Tian-lai1,WU Zhi-gang2,LI Xin1(1.College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture of Liaoning Province,Shenyang 110161,China;2.Institute of Flowers,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China);Effect of Pre-harvest and Post-harvest Calcium Treatments on Muskmelon Softening Physiology[J];Jiangsu Journal of Agricultural Sciences;2009-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved