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《Molecular Plant Breeding》 2015-09
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Analysis on Genetic Relationship of New Breeding Combinations Based on SSR and SRAP Markers in Momordica charantia L.

Wang Guoli;Huang Meihua;Department of Life Science,Huizhou University;Huizhou Agricultural Product Quality Safety Supervision and Inspection Center;  
Genetic relationship analysis of new breeding combinations based on molecular marker technology is essential to screen improved variety in Momordica charantia because of its complicated genetic background. 22 new breeding materials were analyzed based on SSR and SRAP in this paper. 8 pairs of SSR primers and 10 pairs of SRAP primers were screened with high PIC, average polymorphic percentage achieved 72.71% and 83.80%separately in SSR and SRAP amplification results. Cluster analysis showed that both SSR and SRAP could display genetic diversity of all materials efficiently, 2 kind of marker result showed high identity, all materials were divided into 4 classes according to GS value of 0.71 in SSR and 0.64 in SRAP. Complicated 2 markers could uncover the genetic relationship of 22 materials more accurately, all materials were divided in to 5 classes according to GS value of 0.76. BL combination, YS31, JC21 and MY023, farer genetic relationship displayed by SSR and SRAP cluster analysis individually, clustered separately in complicated cluster analysis. 3 groups of HY, GL and ZY combination all clustered into 1 class again, it fit to the fact that HY combination was hybrid from GL and ZY combinations. Results indicated that complicated 2 markers analysis could uncover materials' genetic relationship more accurately in Momordica charantia.
【Fund】: 广东省科技计划项目(2012A020602068)资助
【CateGory Index】: S642.5
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