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《Molecular Plant Breeding》 2015-09
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Test of Variety Purity and Genetic Diversity with SSR Markers for Watermelon New Hybrid Cultivars ‘Kangbing948' and ‘Shenkang988'

Li Chaohan;Liu Li;Liu Xiang;Zhu Lihua;Song Ronghao;Yang Hongjuan;Gu Weihong;Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Lab of Protected Horticultural Technology;Tianjin University;Plant Protection Research Institute,Shanghai Academy of Agricultural Sciences;  
Simple sequence repeat(SSR) molecular markers were used to test variety purity of watermelon hybrids‘Kangbing948'and‘Shenkang988', and were used to analyze genetic diversity of parental germplasms of the two watermelon varities. Two pairs of specific primers(primer BVWS00209 and BVWS00233) out of 38 pairs of SSR core primers were selected to make a rapid variety purity test. The seed purity of‘Kangbing948'and‘Shenkang-988'detected by SSR markers were 97% and 98%, respectively. Compared with results of morphological identification of‘Kangbing948'and‘Shenkang988'in field, the coincidence rate of the variety purity detected by SSR markers was higher than 99%. And the DNA fingerprints of those parental germplasms of ‘Kangbing948'and‘Shenkang988'were constructed by using polymorphic sites of the selected SSR primers. The results showed that the technical system can meet the requirements of rapid variety purity test of the watermelon new hybrids‘Kangbing948'and‘Shenkang988'in laboratory.
【Fund】: 国家科技部农业科技成果转化资金项目(213GB2C000160);; 上海市西甜瓜产业技术体系建设项目(沪农科产字(2015)第1号)共同资助
【CateGory Index】: S651
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