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《Molecular Plant Breeding》 2015-09
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Transgenic With TfdA Gene To Improve the Ryegrass of Herbicide 2,4-D Resistance

Li Cuicui;Han Lizhen;Zhao Degang;Institute of Agro-Bioengineering and College of Life Sciences,The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education;Institute of Agro-Bioengineering and College of Life Sciences,Collaboratiue innovation Center for Mountain Ecology & Agro-Bioengineering,Institute of Agro-Bioengineering and College of Life Sciences;  
2,4-D-degrading gene Tfd A was transformed into shoot apical meristem of Lolium multiflorum Lam by Agrobacterium-mediated transformation. 2,4-D herbicide spraying experiments show that wild type ryegrass became etiolation death after spraying 10 g/L 2,4-D on 17 days later and transgenic plants(transgenic ryegrass with Act1::Tfd A and Act1::Tfd A-Zml3::Flp) still grew well in 2,4-D 10 g/L concentration. The chlorophyll content of all plants were decreased after spraying 10 g/L 2,4-D, the chlorophyll content of all plants were 2.411 mg·g-1·FW,3.006 mg·g-1·FW and 3.039 mg·g-1·FW, reduced by 24.444%, 2.592% and 1.619% than the previous. Expression of Tfd A gene in ryegrass significantly improve the ability to resist the herbicide 2,4-D.
【Fund】: 国家转基因生物新品种培育科技重大专项子课题任务(2014ZX08010-003)资助
【CateGory Index】: S543.6
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Chinese Journal Full-text Database 10 Hits
1 YANG Feng-ping~1,LIANG Rong-qi~1,ZHANG Li-quan~1,ZHANG Xiao-dong~(1*),SUN Zhen-yuan~2(1 Beijing Agro-Biotechnology Research Center,Beijing Agriculture and Forestry Academy,Beijing 100089,China;2 Institute of Forestry Science,Chinese Academy of Forestry,Beijing 100091,China);Perennial Ryegrass Transformed with the Adversity-Resistant Transcription Factor DREB1B Gene[J];Acta Botanica Boreali-Occidentalia Sinica;2006-07
2 Kong Zheng 1 Zhao Degang 1,21 Guizhou Key Laboratory of Agricultural Bioengineering, College of Life Sciences, Guizhou University, Guiyang, 550025; 2 Key Laboratory of Green Pestiside and Bioengineering of Education Ministry, Guiyang, 550025;The Combination of CHI and AFP Genes Introduced into Ryegrass Mediated by Agrobacterium[J];Molecular Plant Breeding;2008-02
3 An Likun;Zhao Degang;Guizhou Key Lab of Agro-Bioengineering, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education, College of Life Sciences, Guizhou University;;Agrobacterium-mediated Genetic Transformation of Maize Shoot Apical Meristem and Detection of Transgenic Maize with TPS1 Gene and VlmybA2 Gene[J];Genomics and Applied Biology;2014-03
4 LI Dong-hua1,2,LIAO Yu-cai1,3,LI He-ping1,2(1.Molecular Biotechnology Laboratory of Triticeae Crops,Huazhong Agricultural University,Wuhan,Hubei 430070,China; 2.College of Life Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China; 3.College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China);Transformation of Shoot Apical Meristems of Elite Barley Cultivars via Agrobacterium-mediated Transformation[J];Journal of Triticeae Crops;2012-01
5 WANG Qi-li1, HE Jin-gang2, CHEN Yan-long2, ZHANG Xiao-yun2, WU Jin-xia2 (1. Shanxi Agricultural University, Shanxi Taigu 030801; 2. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081, China);Establishment of an Agrobacterium Mediated Transformation System for Lolium perenne L.[J];Journal of Agricultural Science and Technology;2009-02
6 HAN Li-zhena,c,ZHAO De-ganga,b,c(Guizhou University a.Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education; b.Guizhou Key Laboratory of Agro-Bioengineering;c.College of Life Science,Guiyang 550025,China);Research Progress of Microbial Degradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid[J];Agrochemicals;2012-10
7 WEI Yan-Hong;MA Ling-Ling;HE Lan-Lan;CHAI Meng-Liang;ZHU Hua-Guo;SUN Jie;ZHANG Wei;Agricultural college, Shihezi University;College of Life Sciences, Shihezi University;;Establishment of Stem Tips Genetic Transformation System Mediated by Agrobacterium in Gossypium barbadense L.[J];Journal of Agricultural Biotechnology;2014-10
8 Li Huaping John Hu Wang Min Fan Huaizhong ( Laboratory of Plant Virology,South China Agriculture University, Guangzhou 510642 China; Department of Plant Pathology, University of Hawaii, Honolulu, HI 96822 USA );Genetic Transformation System of Banana Meristems[J];Chinese Journal of Tropical Crops;2000-04
9 Li Jun1 Li Yan2 Liu Dehu1(1Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081;2College of Life Science,Hebei Normal University,Shijiazhuang 050016);Alternative Methods of Plant Genetic Transformation and Recent Advances[J];Biotechnology Bulletin;2011-07
10 Liu Zhongyu;Zhao Shujin;College of Life Sciences,Yangtze University;General Hospital of Guangzhou Military Command;;Agrobacterium tumefaciens-mediated Transformation of Shoot Tip of Polygonum cuspidatum[J];Biotechnology Bulletin;2014-02
Chinese Journal Full-text Database 10 Hits
1 WANG Zong-wen(Shandong Cotton Research Center,Jinan 250100,China);Research and Application Status of Herbicide-resistant Cotton at Home and Abroad[J];Shandong Agricultural Sciences;2011-01
2 HUANG Sheng-mou (Department of Chemistry and Biology,Xiangfan University,Xiangfan,Hubei 441053);Harm by drought and mechanism of drought resistance of plant[J];Journal of Anhui Agricultural Sciences;2009-22
3 DANG Wei-ling1,SUN Yan1,YANG Qing-chuan2(1.The Department of Grassland Science,China Agricultural University,Beijing 100094,China;2.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100094,China);Advantage of Research on Transgenic Turfgrass[J];Northern Horticulture;2007-08
4 LI Zhi-liang1,2,3,WU Zhong-yi2,YE Jia1,YANG Qing3,ZHANG Xiu-hai2,HUANG Cong-lin2(1.Department of Biology Science,Handan College,Handan,Hebei 056005;2.Beijing Research Center of Agro-Biotechnology,Beijing 100097;3.College of Life Science,Nanjing Agricultural University,Nanjing,Jiangsu 210095);Study Advancements of DREB Transcription Factors and its Application in Plant Stress Tolerance Genetic Engineering[J];Northern Horticulture;2010-20
5 ZHANG Lei1,3,WU Jin-xia1,3,DONG Fang2,3,LIANG Hua1,3,YE Xing-guo2,3,LU Tie-gang1,3,ZHAO Jun1,3(1.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Identification of stress resistant transgenic ryegrass and tall fescue plants expressing ABP9 gene[J];Pratacultural Science;2010-07
6 QIAO Ding-jun1,2,MAO Ping1,2,MA Xin-rong1,YANG Hong1(1.ECORES Lab,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China; 2.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China);The effects of mannitol and AgNO_3 for perennial ryegrass transformation mediated by Agrobacterium tumefaciens[J];Acta Prataculturae Sinica;2011-01
7 ZHANG Xiao-yun1,HE Jin-gang1,2,SUN Xue-hui1,WU Jin-xia1(1.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2.Institute of Genetics and Physiology,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051,China);Transformation of Lolium perenne with a fructan:fructan 1-fructosyltransferase gene from Agropyron cristatum and enhancement of drought tolerance in transgenic plants[J];Acta Prataculturae Sinica;2011-01
8 WANG Zhou1,2,3,LIU Jian-xiu2(1.College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany, Jiangsu Province & Chinese Academy of Sciences,Nanjing 210014,China;3.Biotechnology Institute, Guangxi Academy of Agricultural Sciences,Nanning 530007,China);Advances in studies on DREB/CBF transcription factors,and their applications in genetic engineering for stress tolerance of turf and forage grasses[J];Acta Prataculturae Sinica;2011-01
9 ZHANG Fang,WANG Zhou,ZONG Jun-qin,LIU Jian-xiu,SHE Jian-ming(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Establishment of an Agrobacterium mediated genetic transformation system for Eremochloa ophiuroides[J];Acta Prataculturae Sinica;2011-02
10 CHEN Min,CHEN Ya-ning,LI Wei-hong,CHEN Ya-peng,HE Bin,WAN Jiang-hui (Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China);Drought Resistance Mechanism of Three Plant Species in the Middle Reaches of Tarim River,China[J];Acta Botanica Boreali-Occidentalia Sinica;2007-04
China Proceedings of conference Full-text Database 5 Hits
1 ;不同转基因方法转化石斛兰的研究[A];[C];2006
2 MA Wen-hui~1 ZHANG Qian~(1,2) SHANG Ai-qin~(1,3) ZHAO Liang-jun~1 (1 Department of Ornamental Horticulture and landscape Architecture,China Agricultural University,Beijing 100093; 2 Flower Research Institute,Yunnan Academy of Agriculture Science,Kunming 650205; 3 College of Horticulture,Hebei Agricultural University,Baoding 071001);Studies on Transformation of Euonymus fortunei with P5 CS Gene Cloned from Euonymus japonicus‘Cu Zhi’ via Agrobacterium tumefaciens[A];[C];2009
3 Zhang Miaoxia;Lai Zhongxiong;Huang Yuji;Wu Jinshou Institute of Subtropical Fruits, Fujian Agric. and Forestry Univ, Fuzhou 350002;Efficient Plant Regeneration of Banana (Musa spp.)using Thin-cell-layer Culture[A];[C];2004
4 Yao Na~1 Han Liebao~(1,2*) Zeng Huiming~1 Xu Bing (1.Turfgrass Institute of Beijing Forestry University,Beijing 100083; 2.College of Horticulture and Gardening of Yangtze University,Jingzhou Hubei 430025);Application and development of turfgrass breeding transgenic technology[A];[C];2007
5 LI Huaping, ZhOU Guohui, XU Chunxiang, RAO Xueqin (Laboratory of Plant Virology, South China Agricultural University, Guangzhou 510642);Transformation of the Coat Protein Gene of Cucumber Mosaic Virus In Banana[A];[C];2002
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Fu-yong(College of Life Science,Yangtze University,Jingzhou,Hubei 434025);Establishment of Agrobacterium-mediated Transformation System of Cotton Shoot Tip[J];Journal of Anhui Agricultural Sciences;2009-02
2 Li Huaping;John S Hu;Kelly Barry; Fan Huaizhong(South China Agricultural University, Guangzhou 510642)The plant expression vectors of the CP genes of Cucumber mosaic virus (CMV)strains in -ecting banana were constructed, and were used to transform;STUDIES ON TRANSGENIC TOBACCO PLANTS WITH THE COAT PROTEIN GENE OF CUCUMBER MOSAIC VIRUS STRAINS INFECTING BANANA[J];Chinese Journal of Virology;1996-02
3 YANG Fu yu, ZHOU He (Institute of Grassland Science, China Agricultural University, Beijing, 100094, China);Review on Salinity Tolerance of Turfgrass[J];Grassland and Turf;2001-01
4 WU Gui-sheng1,2,3,XU Er-ni3,SONG Fu-ping2,HU Yuan-lei1(1.College of Life Science,Beijing University,Beijing 100871,China;2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China;3.The Key Laboratory of Food Science of MOE,Nanchang University,Nanchang 330047,China);Biological Control of Insect Pests in turfgrass[J];Pratacultural Science;2007-06
5 WU Jin-xia,CHEN Yan-long,HE Jin-gang,LU Tie-gang(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Biotechnology and its application on quality improvement for forage grasses[J];Acta Prataculturae Sinica;2007-01
6 ZOU Li1,2,WEN Yi2,ZHANG Yun-hua1,WANG Dan2(1Institute of Plant Protection,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;2 Northeast Forestry University,Harbin 150040,Heilongjiang,China);Susceptibility of Soybean Cotyledonary Node to Hygromycin B[J];Soybean Science;2008-02
7 YU Shu-hong,ZHAO Li-li,WANG Wei,ZHANG Di-qun~*(College of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China);Factors Affecting High-frequency Induction of Hair-like Roots of Reynoutria Japonica Houtt.[J];Acta Botanica Boreali-occidentalia Sinica;2005-09
8 MA Zhong hua, ZHANG Yun fang, XU Chuan xiang,CHEN Wen Jun, YING Hong hua, KUAI Ben ke (Department of Biochemistry);Tissue culture and Genetic Transformation of Kentucky Bluegrass ( Poa pratensis) via Microprojectile Bombardment[J];JOURNAL OF FUDAN UNIVERSITY;1999-05
9 Yang Bo Ding Liping Yao Lu He Guangyuan Wang Yuesheng China-UK HUST-RRes Crop Genetic Engineering and Genomics Joint Lab, Huazhong University of Science and Technology (HUST), Wuhan, 430074;Effect of Seedling Ages and Inoculation Durations with Agrobacterium Tumefacien on Transformation Frequency of the Wheat Wounded Apical Meristem[J];Molecular Plant Breeding;2008-02
10 He Yating 1 Qu Yanying 1 Kong Qingping 2 Yan Yongliang 1 Shang Shuang 1 Zheng Liangyu 1 Chen Quanjia 11 Agriculture College of Xinjiang Agriculture University, Urumqi, 830052; 2 Institute of Cash Crops Research, Xinjiang Academy of Agricultural Sciences, Urumqi, 830000;Primary Studies on the Effects of Callus Induction and Differentiation in Gossypium Barbadense[J];Molecular Plant Breeding;2008-03
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