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《Molecular Plant Breeding》 2015-10
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Modification of‘Gene-Deletor'Vector with Tomato Fruit-specific Promoter E8 and Transformation of Kumquat

Xie Yuming;Li Yi;Yan Jiawen;Yao Runxian;Alessandra Gentile;Deng Ziniu;College of Horticulture and Landscape,HNAU;Changsha Branch of National Citrus Improvement Centre;  
In order to solve the edible safety problem of transgenic kumquat and get the non-genetic food from transgenetic crop,the foreign genes were removed from the fruit of the transgenic kumquat by using‘gene-deletor'technology which has been proven effectively in some transgenetic plants.Here,we cloned fruit specific promoter E8 from tomato and modified ‘gene-deletor'vector with E8 promoter,in which E8 promoter replace PAB5 promoter to drive recombinase FLP gene,Ca MV35 S promoter to drive report and selecting gene GUS::NPTⅡ.The seedling internode stem segments of kumquat(Fortunella crassifolia Swingle cv.Jindan) were transformed with the‘gene-deletor'vector mediated by Agrobacterium tumefaciens.We obtained 11 resistant plants and 6 transformed plants confirmed by PCR analysis.The transformed plants will be tested for the gene-deletor function later on.The improvement application of‘gene-deletor'technology in kumquat provided an alternative for citrus safety transgenic research.
【Fund】： 国家自然青年基金项目(31201596);; 湖南省科技计划项目(2014NK3014)共同资助
【CateGory Index】： S641.2
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