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《Molecular Plant Breeding》 2015-10
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Clonging and Subcellular Location of a DREB Transcription Gene of Paeonia suffruticosa

Liu Huichun;Ma Guangying;Zhu Kaiyuan;Zou Qingcheng;Zhou Jianghua;Zhang Jiaqiang;Research & Development Center of Flower,Zhejiang Academy of Agricultural Sciences;  
DREB transcription factor is a dehydration responsive element binding protein,which plays a very important role in regulating its molecular response in plants to drought,high salt and cold stress.In this research,a full-length cDNA of DREB gene from Paeonia suffruticosa‘Luo Yanghong'leaves was obtained and named Ps DREB.The cDNA sequence was 1 661 bp,which contained 1 089 bp open reading frame for 363 amino acid residues,448 bp 5'-terminal and 124 bp 3'-terminal sequence.Homology analysis indicated that the Ps DREB protein showed 61%,60%,56% and 55% similarity to the DREB homologs from Vitis vinifera,Theobroma cacao,Populus trichocarpa and Coffea Arabica.In order to further verify the function of Ps DREB,subcellular localization analysis was carried out by gene gun method.The results showed that the PsDREB protein was localized in the nucleus,indicating that the protein was consistent with the characteristics of nuclear localization of transcription factors.These results formed a basis to further study the structure and function of the gene encoded protein.
【Fund】: 浙江省自然科学基金项目(LQ13C150003);; 浙江省农科院创新能力提升工程项目共同资助
【CateGory Index】: S685.11
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