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《Molecular Plant Breeding》 2018-03
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SNP Analysis of Wild and Cultivated Cannabis Based on Whole Genome Re-sequencing

Chen Xuan;Guo Rong;Wang Lu;Liu Yanhu;Guo Mengbi;Xu Yanping;Guo Hongyan;Yang Ming;Zhang Qingying;Industrial Crop Research Institute of Yunnan Academy of Agricultural Sciences;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University;  
To reveal the differences between genomes of wild and cultivated Cannabis, we used whole genome resequencing technology to re-sequence one wild(ym606) and one cultivated(ym224-B) Cannabis with 10× depth.2 264 150 single nucleotide polymorphisms(SNPs) had been detected by comparing with the reference genome(Cansat3_genome). The heterozygosity rates of ym606 and ym224-B were 0.12% and 0.11%, respectively. The two-level polymorphism SNPs between two individuals were divided into 4 categories, which were aa ×bb, lm×ll,nn×np and hk×hk. The number of aa×bb type markers was 988 575, accounting for 46.32% of the total number of SNPs, and lm×ll, nn×np and hk×hk accounted for 25.17%, 22.59% and 5.71%, respectively. The results reflected the difference between genomes of wild and cultivated Cannabis in China, which might provide theoretical basis and reference for the construction of genetic segregation populations of wild and cultivated Cannabis and the development of molecular markers for some significant characters.
【Fund】: 国家自然科学基金项目(31660407;31360350);; 云南省重点新产品开发专项(2016BB005)共同资助
【CateGory Index】: S563.3
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