Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2018-06
Add to Favorite Get Latest Update

Preliminary Research on Potato Dual-antiviral Transgenic Technology

Zhang Xiying;Zhang Aiping;Liu Jiangna;Xinjiang Agricultural Science Research Institute;  
According to the viral degeneration and biosafety problems of transgene itself in potato production,RNA interference technology and marker free transgenic technology which have been developed rapidly were introduced into plant antiviral genetic engineering. Then, biological safety type anti virus transgenic potato was cultivated and a biosafety transgenic new pathway with multi-resistance or even multiple immunization was explored. Mediated by Agrobacterium tumefaciens, RNA interference gene structure of against potato virus V and virus Y was transferred into referee cultivated varieties in Xinjiang, including potato Atlantic and the new No. 1.After PCR detection, high biosafety transgenic potato plants without marker gene which had double high resistance or immunity to virus X and virus Y were obtained. Finally, through several times of screening test, it was determined that the Agrobacterium OD600 was advisable between 0.3 and 0.5, with the optimal at 0.5. The best transformation condition was preculture for 2 d, infection for 10 min, and co-culture for 2~3 d. Through specific primer PCR amplification, part of the regeneration plant obtained the same target bands as the positive control group, preliminarily indicating that RNAi gene had been integrated into the potato genome. The target gene of thevirus resistant marker free transgenic potato was of RNA interference type structure, and the resistance of transgenic plants was the result of RNA silencing. Because the transgenic m RNA had been degraded into small fragments, there was no marker gene in transgenic plants, which avoided the potential biosafety problems of target genes and marker genes.
【Fund】: 五家渠市科技计划项目(1506)资助
【CateGory Index】: S435.32
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved