Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2018-10
Add to Favorite Get Latest Update

Cloning, Expression and Bioinformatic Analysis of Cu/Zn-SOD Gene in Rice

Luo Ying;Xie Min;Zhang Chao;Wang Weiping;Zhu Jianhua;Wang Qiming;Rao Liqun;College of Bioscience and Biotechnology, Hunan Agricultural University;College of Chemistry and Biological Engineering,University of Science and Technology;State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center;  
In order to explore the function of Cu/Zn Superoxide Dismutase Gene(OsCu/Zn-SOD) in rice, OsCu/Zn-SOD was obtained by homology cloning method, and was further carried out with expressions and bioinformatic analysis with Oryza sativa as the material. The result showed that the cDNA sequence of OsCu/Zn-SOD was 606 bp, encoding a protein of 152 amino acids. The CDS region of OsCu/Zn-SOD was 459 bp whose compositions of basic group were 22.7% of A, 24.4% of T, 29.4% of G and 23.5% of C. The OsCu/Zn-SOD protein was stable, hydrophilic and mildly acidic, with random coil and extended strand as the main components in secondary structure, and the 3 D model was successfully constructed, and spatial structure was mainly the spiral and the rotation angle. Subcellular localization of OsCu/Zn-SOD was mainly distributed in the cytoplasm and nuclei without transmembrane region and signal peptide, which OsCu/Zn-SOD mainly played a role in the processes of translation, transport and binding, and energy metabolism. The protein sequence included eight serinephosphorylation sites and fou r threonine phosphorylation sites. Further phylogenic analysis displayed that the cloned OsCu/Zn-SOD amino acid sequence had higher conservatism which showed close genetic relationship with Zea mays and Brachypodium distachyon, and far genetic relationship from Physcomitrella patens. The RT-PCR analysis indicated that the Cu/Zn-SOD gene expressed in all tissues. The highest expression levels were found in stem, followed by the leaves and tassel, respectively, with the lowest expression in the root. The study might provide important help for the further study on the role of OsCu/Zn-SOD in rice resistance.
【Fund】: 国家自然基金(31301081);; 科技部国家科技支撑计划项目(2014BAD01B04);; 教育部博士点基金项目(20134320120009);; 湖南省高校创新平台开放基金项目(13K063);; 湖南省自然科学基金项目(2018JJ2144);; 湘南优势植物资源综合利用湖南省重点实验室资助项目(湘南植物[2015]1号)共同资助
【CateGory Index】: Q943.2;S511
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved