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《Molecular Plant Breeding》 2018-10
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Cloning, Localization and Expression Analysis of Epidermal Pattering Factor-like Gene(TaEPFL1) in Wheat

Sun Qingxu;Qu Jipeng;Peng Zhengsong;Yu Yan;Yang Jun;Wu Yilei;Yang Zaijun;Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education),College of Life Science, China West Normal University;School of Agricultural Science, Xichang University;  
Members of the epidermal pattering factor-like(EPFL) family participate in regulating a series of growth and development in plant, including the control of stomatal density and guidance of pollen tube elongation. In this work, we identified and characterized an EPFL gene(TaEFPL1) in wheat pistillody mutant HTS-1. Sequencing results indicated that this gene consisted of three homologous genes(TaEFPL1.1, TaEFPL1.2, and TaEFPL1.3).Also, the wheat nulli tetrasome analysis(NT) showed that these 3 homologous genes were located at chromosome6 D, 6 B and 6 A. The open reading frame(ORF) of the three homologous genes was 363 bp, encoding 120 amino acids. The ORF sequences of TaEPFL1.2 and TaEPF1.3 were the same. The phylogenetic analysis indicated that TaEPFL1 belonged to the EPFL family in plant, and that it was closely related to ZmEPFL1, ObEPFL1, and AtEPFL1. Real-time PCR revealed that the TaEPFL1 gene showed an abnormally high expression in pistillody stamens in HTS-1. Therefore, we deduced that the overexpression of the TaEPFL1 gene might contribute to the homeotic transformation of stamens into pistils or pistil-like structures. These data provided a basis for the futureresearch on the function of TaEPFL1 gene and the molecular mechanism of the transformation of stamen homologous to pistil in wheat.
【Fund】: 四川省科学技术厅基金会(16JC0022);; 四川省教育厅创新团队项目(16TD0020);; 四川省青年科技创新团队(2017TD0008)共同资助
【CateGory Index】: Q943.2;S512.1
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