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《Molecular Plant Breeding》 2018-10
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Cloning and Expression Analyzing of a Male Sterile Related Gene ms2 of Lycium barbarum L.

Cheng Hui;Fu Haihui;Zhou Jun;Cao Youlong;Li Yanlong;Qin Ken;Dai Guoli;Duan Anan;Duan Linyuan;Forestry College, Southwest Forestry University;Center of Chinese Wolfberry Engineering and Technology;Beifang University of Nationalities;  
Specific primers were de signed according to the conserved sequences of ms2 genes in Gen Bank and fragment of ms2 genes specific to male sterility were obtained by amplifying genomic DNA of the male fertile line of Lycium barbarum L. the primers, and 3'c DNA was cloned using RACE technology. The fragment of the gene was 1 808 bp in length by joint, which was submitted to Gen Bank under accession number JQ341412.1, contained the ending code-TAG, the fragment of 3'-terminal and Poly A tail. Its corresponding encoded 527 amino acids. In bio-information analysis, the corresponding peptide segment contained NAD(P) H-binding protein. It typically had a TGXXGXXG cofactor binding motif and microbody targeting signal. There was NAD(P) H-binding from 44 amino acid to 346 and N-terminal of male sterility from 459 amino acid to 517. Through Blastp program, the ms2 protein from Lycium barbarum L. were found sharing 71%, 71%, 72%, 65%, 66% identity with the known ms2 proteins from Linum usitatissimum, Vitis vinifera, Glycine max, Arabidopsis thaliana and Brassica chinensis, respectively.In phylogenetic analysis, the ms2 gene had closely relativeship with grape, which was clustersed in a group with Populus trichocarpa, Linum usitatissimum and Cruciferae, and had distantly relativeship with Moellendorf's Spikemoss Herb. RT-PCR revealed that the ms2 gene expressed strongly in flower buds of stage Ⅱ and stage Ⅲ of the male fertile lines, while expressed weakly in flower buds of stage Ⅱ and stage Ⅲ from the male sterility lines.
【Fund】: 国家自然科学基金(30760127;31360360;31260190);; 宁夏回族自治区育种专项(2013NYYZ0102)共同资助
【CateGory Index】: Q943.2;S567.19
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