Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2018-10
Add to Favorite Get Latest Update

Cloning, Subcellular Localization and Expression Analysis of PpSPL4 Gene in Poa Pratensis

He Chunyan;Yang Qingqing;Liu Haijin;Yin Shuxia;Research Institute of Beijing Forestry University;Beijing Institute of Education;Affiliated Middle School of Beijing College of education;  
In this experiment, the PpSPL4 gene was obtained by homologous cloning. The results showed that the PpSPL4 gene contained the conserved region of SBP gene family, the similarity of which with SBP gene in other plants was more than 67.83%. Its open reading frame was 961 bp, encoding 327 amino acids, and the isoelectric point was 9.07. Advanced structure analysis indicated that the PpSPL4 gene had the highest similarity with AtsSPL4 gene; subcellular localization results suggested that the protein encoded by this gene located in the nucleus. The expression analysis of PpSPL4 gene in rhizomes, roots, stems, leaves and different flowering stages of Poa pratensis demonstrated that the relative expression of PpSPL4 gene was different in different tissues of Poa pratensis, with the highest expression in flower. Meanwhile, PpSPL4 gene was induced by GA and sucrose,suggesting that the gene might be the transcription factor involved in the flower bud differentiation and GA pathway during the flower development of Poa pratensis. The study of PpSPL4 gene could provide technical theories for mechanism research and breeding of Poa pratensis.
【Fund】: 国家自然科学基金项目(31302016)资助
【CateGory Index】: Q943.2;S688.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved