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《Molecular Plant Breeding》 2018-10
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Agrobacterium-mediated Genetic Transformation of Alfalfa and Salt-tolerant of PDH45 Transgenic Alfalfa

Jin Taicheng;Liu Yuqing;Xu Yanan;Meng Dawei;Zhou Xiaomei;Yang Liping;School of Life Science, Jilin Normal University;Key Laboratory for Plant Resources Science and Green Production;  
The genetic transformation o f salt-tolerant genes can improve the ability of plants to resist salt stress and effectively solve the inhibition of salt stress to plants. In this research, Medicago sativa was used as the plant materials, and PDH45 gene was transformed into Medicago sativa by using the method of Agrobacterium-mediated leaf disc transformation. A variety of conditions influencing efficiency of genetic transformation were optimized,including Agrobacterium bacteria concentration, Agrobacterium infection time, kanamycin concentration of the selective medium. PDH45 transgenic alfalfa was tested by using the semi-quantitative RT-PCR, and the results showed that PDH45 genes were all expressed in the transgenic plants. Under the Na Cl concentration of 50 mmol/L,100 mmol/L, 150 mmol/L and 200 mmol/L, the study detected the salt-tolerant of PDH45 transgenic alfalfa, and the experimental results proved that PDH45 transgenic plants had strong tolerance to moderate salt stress of150 mmol/L Na Cl. This study rapidly obtained PDH45 transgenic alfalfa by optimizing experiment condition, and would provide research base for the application and spread of salt-tolerant transgenic alfalfa.
【Fund】: 吉林省教育厅“十三五”科研规划项目(JJKH20180794KJ)资助
【CateGory Index】: S541.9
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