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《Molecular Plant Breeding》 2018-10
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Molecular Detection of Rice Blast Resistance Gene in the Main Rice Germplasms in Henan Province

Wang Ya;Chen Xiangong;Yin Haiqing;Wang Yuetao;Yang Ruifang;Zang Zhiguang;Wang Shengxuan;Institute of Food Crops, He'nan Academy of Agricultural Sciences;Shanghai Nongke Seeds and Seedlings Company Limited;  
The aim of this study was to be clear about the distribution of six blast resistance genes Piz-t, Pikm, Pit,Pi25, Pid2 and Pid3 in the main rice germplasms in He'nan province, so as to provide reference for the breeding of new varieties with rice blast resistance in He'nan using molecular marker-assisted selection. In this study, the specific molecular markers based on the sequence difference between the blast resistant gene and its susceptible allele was designed to detect the blast resistance genes of 140 He'nan rice germplasms. The results showed that the distribution of blast resistance genes in these materials was less as a whole, and most of them contained 1~2 resistant alleles. Of these six detected genes, Piz-t was the most widely distributed, followed by Pikm and Pid3;Pid3 was relatively few, while Pit and Pi25 were the least. The blast resistance genes contained in the rice varieties from Henan(87) were less than those in the introduced varieties(53), which showed that 10.3% of the former mat-er ials and 32.1% of the latter materials were found to contain more than 3 resistant alleles. Except Piz-t and Pikm, the gene frequency of Pit, Pi25, Pid2 and Pid3 varied greatly in different detecting groups. This study could provide theoretical basis and guidance for the rice breeding of broad-spectrum and durable blast resistance in He'nan.
【Fund】: 河南省农业科学院优秀青年科技基金(2018YQ04);; 河南省重大科技专项(171100110300);; 河南省水稻产业技术创新团队首席专家项目(S2010-04)共同资助
【CateGory Index】: S435.111.41
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