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《Molecular Plant Breeding》 2018-10
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Truth Identification of F_(1:2) Plants by SSR Markers in Foxtail Millets

Li Tao;Ren Qinyong;Li Qiang;Yang Wenyao;Guo Shihua;Agricultural College, Inner Mongolia Agricultural University;Crop Institution, Inner Mongolia Academy of Agricultural and Animal sciences;  
In order to rationally use millet hybrid F_(1:2) plants, parents combined by low amylose content×waxy millet and their hybrid F_(1:2) plants were used as materials to identify the truth of F_(1:2) plants by morphology and SSR molecular markers. The results showed that female parent of 2015(07) combination was conical tight spike, while the male parent was cat claw loose spike. 2015(07)-4 was true hybrid associated with cat claw tight spike, and complementary bands of their parents in SSR markers, and the other three plants were false hybrid with conical tight spike, same as the mother belt in SSR markers. Parents of 2015(13) combination had conical spikes, dark green stem and leaves, and F_(1:2) was undistinguishable in morphology. 17 pairs of better polymorphism primers between parents were screened from 40 pairs of SSR primers in 2015(13) combination. 13 F_(1:2) plants from 2015(13)-1 to2015(13)-13 were detected for PCR amplification using these 17 paris of primers, the result of which showed that only 2015(13)-6 and 2015(13)-9 were false hybrids, and their SSR belt types were same as the mother belt. The other11 plants were true hybrids owing to their SSR belts were complementary bands of their parents in SSR markers.F_(1:2) plants fidelity frequency of the combination of 2015(07) and 2015(13) were 25.0% and 84.6%, respectively.In this study, the authenticity of the F_1 generation of millet was identified by field observation combined with SSR molecular markers, which provided experimental evidence for F2:3 generation population selection and individual plant selection.
【Fund】: 国家谷子高粱产业技术体系种子繁育与质量控制(CARS-06-13.5-A15);; 国家自然科学基金项目(31560394)共同资助
【CateGory Index】: Q943.2;S515
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