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《Molecular Plant Breeding》 2018-10
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Establishment of High Efficient Identification System for Alien Addition Lines of B Genome in Brassica

Tan Chen;Zhu Kaiyuan;Xiang Yi;Li Zaiyun;Research and Development Centre of Flower, Zhejiang Academy of Agricultural Sciences;National Key Lab of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University;  
Brassica B genome carries many useful genes and traits for breeding, but the studies on it are quite limited. To dissect the B genome by developing of a complete set of B. oleracea-nigra MAALs(monosomic alien addition lines), we created an efficient, rapid and accurate identification and screening system for the Brassica B genome chromosomes with SSR molecular markers and FISH as the core in this study. Using this system, we identified and screened MAALs in the backcross progenies of that the triploid F1 hybrid(2 n=26, CC.B) was used as the maternal parent and backcrossed successively to parental B. oleracea(2 n=18, CC). Results showed that the MAALs with each of eight different B-genome chromosomes added to C genome(2 n =19, CC+1 B1-8) could be accurately identified and screened by this identification and screening system. Moreover, the efficiency was very high that seven different MAALs were established just within 3 backcross generations. The MAALs of B. oleraceanigra would be valuable for exploiting the genome structure and evolution and using the useful traits of Brassica B genome.
【Fund】: 国家自然科学基金(31371656)资助
【CateGory Index】: Q943.2
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