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《Molecular Plant Breeding》 2018-10
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A New Method for Rapid Extraction of RNA in Kiwi

Deng Lang;Bao Changyan;Zhou Jun;Wang Lianchun;Liu Huimin;Ji Qing;Shen Bingqi;Zhou Fan;Wang Dawei;Key Laboratory of Biodiversity Conservation of the State Forestry Administration of Southwest China;Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of Education, Southwest Forestry University;College of Biological Sciences and Engineering, North Minzu University;  
In this study, the total RNA was extracted from young leaves, flowers and young fruits of‘Hort-16 A'by reference to CTAB method, guanidine thiocyanate method, trizol method, SDS method and"rapid separation of high quality RNA from seed endosperm containing high levels of starch"method, and the results of the reference methods were compared. After the improvement of the above methods, the method of rapid, stable, simple operation and low cost was selected which was suitable for the total RNA extraction of kiwifruit. Compared with several methods referenced, RNA extracted in the new method was integrated and clarified. Using the reverse transcription c DNA of extracted RNA as template, two pairs of specific primers designed from AP2 gene and AG gene were applied for RT-PCR amplification. The stripe obtained was bright, single and complete, which was the required purpose strip after sequencing. The RNA extracted by this new method might provide data information for the next step in gene cloning and functional verification experiments.
【Fund】: 国家林业局948项目(2012-4-62)资助
【CateGory Index】: S663.4
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