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《Molecular Plant Breeding》 2018-10
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Embryogenic or Not Callus Induction and Analysis of Physiological and Biochemical Differences in M. wufengensis

Ning Nana;Deng Wei;Zhu Zhonglong;Sang Ziyang;Chen Faju;Ma Lvyi;Jia Zhongkui;College of Forestry, Beijing Forestry University;Biotechnology Research Center, China Three Gorges University;Wufeng Boling Magnolia wufengensis Technology Development Co. Ltd.;  
In order to obtain embryogenic callus with good growth status, the embryogenic callus(EC) were inducted from immature seeds of Magnolia wufengensis which were used as the explant. The seeds were cultured in 1/2 MS minimal medium with 2,4-D, 6-BA and casein hydrolysate(CH) in different concentrations, and then sucrose concentration was improved and abscisic acid(ABA) was added for better growth. Besides, soluble protein, soluble sugar and free proline of embryogenic callus were determined for differential analysis. The results showed that the content of the optimal induction medium was 1/2 MS, 2.0 mg/L 2,4-D, 0.2 mg/L 6-BA and 750 mg/L CH(induction rate up to 96.8%). Embryogenic callus grew well when they were transferred to medium with sucrose concentration increasing to 5% and 6% and the induction rate could reach 50.0% and 64.7%, respectively.Meanwhile, the soluble protein and free proline content of embryogenic callus were significantly higher than other treatments. Especially when the sugar concentration was 6%, the soluble sugar content was significantly higher than other treatments. This phenomenon indicated that embryogenic callus had higher metabolic activity than non-embryogenic callus(NEC). The embryogenic callus treated with higher concentration of sucrose had a positive response to this stimulus, which could provide a scientific basis for the differentiation of somatic embryos.
【Fund】: 林业公益性行业科研专项(201504704)资助
【CateGory Index】: S685.15
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