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《Molecular Plant Breeding》 2018-10
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Optimization of DNA Extraction Condition of Hainan Melastoma L. Based on Response Surface Methodology

Zhao Furui;Wang Mengjie;Gao Xiaoxin;Liu Xia;Yang Weili;Pharmaceutical College, Hainan Medical University;  
In order to study whether Melastoma L. in different environments were the same ecotypes, and analyze the interspecific relationship of Hainan Melastoma, the study established and optimized the DNA extraction conditions of Melastoma in Hainan Province, and used the DX8 Trial software to improve the CTAB concentration,PVP concentration, β-mercaptoethanol concentration, sodium acetate content and other factors. The results showed that the optimum extraction conditions were as follows: 2% CTAB, 2% PVP, 1% β-mercaptoethanol and 5 mol/L sodium acetate 90 μL. Meanwhile, using this condition to extract the DNA of other plants in Melastoma,the result showed higher DNA purity and clear bands as well as good extraction effect. At last, the extraction condition was applicable to the extraction of the plant DNA of Hainan Melastoma, and might provide a basis for the study of genetic diversity of Melastoma plant, and molecular pharmacognosy.
【Fund】: 海南医学院大学生创新训练计划项目(No.HYCX2015012);; 2016年地方高校国家级大学生创新创业训练计划项目(No.201611810067)共同资助
【CateGory Index】: Q943.2;S685.11
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